Antioxidant and cytotoxic activity of Ampelopsis brevipedunculata and Parthenocissus tricuspidata

The antioxidative activity and cytotoxicity of the methanol extracts of leaves and inflorescence of two climbing plants species, Ampelopsis brevipedunculata and Parthenocissus tricuspidata (Vitaceae), were studied. Both species originate from East and Southeast of the Euroasian continent, and were introduced to Serbia as an horticulture species. Stilbene derivatives and phenylpropanoid compounds were isolated from the roots of A. brevipedunculata and from the stem wood and leaves P. tricuspidata, as well caffeic acid derivatives from the leaves of P. tricuspidata [1–4].

The methanol extract of inflorescence P. tricuspidata exhibited the highest effect in DPPH test with an IC₅₀=7.55±0.07µg/ml, while the methanol extract of the inflorescence of A. brevipedunculata showed inhibitory effect s on lipid peroxidation with an IC₅₀ value of 33±0.001µg/ml.

The tested extracts exhibited significant in vitro cytotoxic effect on the estrogen-nondependent breast cancer cell line MDA-MB-453. The highest activity was shown by the methanol extract of the leaves P. tricuspidata with an IC₅₀=56.76±7.11µg/ml. The methanol extracts of A. brevipedunculata did not show any cytotoxic activity on the estrogen-dependant breast cancer cell line MDA-MB-361.

References: 1. Oshima, Y., et al. (1993) Phytochemistry 33: 179–182. 2. Inada, A. et al. (1991) Chem. Pharm. Bull. 39: 2437–2439

3. Kim, H. et al. (2005) Planta Med. 10: 973–976. 4. Saleem, M. et al. (2004) Arch Pharm Res. 27: 300–304