Antimicrobial activities of the flower extract of Celosia cristata L
Aim: To evaluate the antimicrobial properties of ethanolic, methanolic and other solvent extracts of Celosia cristata L. used in Korean oriental medicine against microorganisms, Staphylococcus aureus, Bacillus subtillus, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa and Candida albicans.
Methods and Results: The minimal inhibitory concentration (MIC) values of the extracts against animal pathogenic bacteria and yeast were assessed using the broth microdilution methods (M27-A2, M07-A7) . These broth dilution methods M27-A2, M07-A7 standardized by the National Committee for Clinical Laboratory Standards (NCCLS) allowed to determine the MIC of different extracts. Results showed that the different extracts differed clearly in their antimicrobial activities. Hexane fraction of methanolic and ethanolic extracts exhibited good activity against Staphylococcus aureus (0.125mg/mL), Bacillus subtillus (0.5mg/mL) and Candida albicans (1mg/mL) and dichloromethane fraction showed similar results. Quality controls were carried out using amoxicillin, ciprofloxacin, and amphotericin B as standard antibiotics
Conclusions: Celosia cristata L. extracts had the ability to inhibit bacterial growth. These antimicrobial activities differ with the applied extractive solution such as methanol, ethanol and other solvent (hexane, ether, dichloromethane, ethyl acetate, butanol). Hexane and dichloromethane fraction showed a good results MIC values in the range of 0.125 to 1mg/mL
References: 1. National Committee for Clinical Laboratory Standards (NCCLS). (2002) Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard-second edition, NCCLS document M27-A2 (ISBN 1–56238–469–4). NCCLS, 940, West Valley Road. Suite 1400, Wayne, Pennsylvania, 19087–1898, USA.