Antioxidant properties of some Turkish medicinal plants

I. I. Tatli; S. Sahpaz; F. Martin-Nizard; A. Sezgin; O. Arısan; B. Gressier; F. Bailleul; N. Ezer

doi:10.1055/s-0028-1084009

Planta Medica, Table of Contents

Antioxidant properties of some Turkish medicinal plants

II Tatli ¹, S Sahpaz ², F Martin-Nizard ³, A Sezgin ¹, O Arısan ¹, B Gressier ⁴, F Bailleul ², N Ezer ¹

¹Hacettepe University, Faculty of Pharmacy, Dept. of Pharmaceutical Botany, Sıhhiye, TR-06100 Ankara, Turkey
²Université de Lille 2, Faculté de Pharmacie, Laboratoire de Pharmacognosie (E.A. 1043), B.P. 83, F-59006 Lille, France
³Université de Lille 2, Faculté de Pharmacie B.P. 83, 59006 Lille cedex; Faculté de Médecine, Laboratoire JK, 59035 Lille cedex; INSERM U 545, 59019 Lille cedex; Institut Pasteur de Lille, 59019 Lille cedex, France
⁴Université de Lille 2, Faculté de Pharmacie, Laboratoire de Pharmacologie et de Pharmacie Clinique, B.P. 83, F-59006 Lille, France

Abstract

There is a growing interest in oxygen-containing free radicals in biological systems and their implied roles as causative agents in the aetiology of a variety of chronic disorders. The present investigation is a preliminary in ongoing research on plants used in Turkish traditional medicine for their antioxidant activity. These plants mentioned in Table are used for haemorrhoids, abdominal pains and wound healing in traditional Turkish medicine [1,2]. The radical scavenging activities of the methanolic extracts prepared from the aerial parts of these plant species growing in Turkey were evaluated against reactive oxygen species such as superoxide anion (O₂·-) and hydrogen peroxide (H₂O₂) in a non-cellular system as well as versus DPPH radical. Another goal in this study was to test whether methanolic extracts inhibited Cu⁺²-induced low-density lipoprotein peroxidation. These extracts exhibited dose-dependent inhibition on DPPH radical, as well as Cu⁺²-induced LDL peroxidation, and the results indicated that all extracts were effective on non-cellular antioxidant systems of O₂·- when compared with the H₂O₂ group. The results are given in Table.

Table. Antioxidant activities of methanolic plant extracts

Plant name	ED₅₀ (mg/L)
	DPPH	O₂ ^.-	H₂O₂	LDL
Hypericum orientale	17.0±1.5	295.1±18.5	309.5±26.9	17.3±2.1
Helichrysium plicatum subsp. plicatum	39.0±4.8	305.2±15.9	301.6±32.6	36.5±4.6
Centaurea drabifolia subsp. detonsa	59.0±5.2	430.6±25.2	583.6±41.9	54.4±5.0
Centaurea drabifolia subsp. drabifolia	80.0±6.1	290.2±15.5	310.5±18.9	71.0±5.8
Achillea wilhelmsii	46.0±4.0	435.2±32.0	>2000	34.2±3.6
Rubus canescens var. canescens	10.0±1.1	283.8±12.0	1740±120	13.1±2.1

References: 1. Ezer, N., Mumcu Arısan, O. (2006) Turk. J. Botany 30: 223–230.

2. Sezgin A (2005) Folk Medicines in Suhut (Afyon), Hacettepe University, Health Sciences Institute, Ms Thesis, Ankara.