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Supercritical carbon dioxide extraction of bioactive flavonoids from Strobilanthes crispus
The flavonoid compounds of Strobilanthes crispus obtained from supercritical fluid extraction, were investigated and the obtained crude extract yields were compared in order to select the best operation parameters. Since the carbon dioxide was a non polar solute, ethanol was used as co-solvent to increase the polarity of the fluid. The studied parameters in this work included: pressure (100bar, 150bar and 200bar), temperature (40oC, 50 oC and 60 oC) and time extraction (40min, 60min and 80min). The highest extraction yield was obtained at 200bar, 60 oC and 80min. Based on the overall extraction curves, pressure had predominant effect on the extraction yield. The obtained product under optimum SFE conditions and additionally two other SFE conditions including minimum (100bar, 40 oC and 40min) and maximum (200bar, 60 oC and 80min) level of each studied parameter were analysed by HPLC to determine the major bioactive flavonoids from Strobilanthes crispus.