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CC BY 4.0 · Endosc Int Open
DOI: 10.1055/a-2697-7599
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Endoscopic indigo carmine spraying for evaluation of intestinal mucosal permeability: A prospective pilot study

Hirokazu Fukui
1   Department of Gastroenterology, Hyogo Medical University, Nishinomiya, Japan (Ringgold ID: RIN12818)
,
Shojiro Kikuchi
2   Department of Omics Medicine, Hyogo Medical University, Nishinomiya, Japan (Ringgold ID: RIN12818)
3   Institution for advanced Medical Sciences, Laboratory of Medical Frontiers, Hyogo Medical University, Nishinomiya, Japan (Ringgold ID: RIN12818)
,
Noriyuki Ojima
4   Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan (Ringgold ID: RIN65828)
,
Tomonori Yokoyama
4   Analytical and Measuring Instruments Division, Shimadzu Corporation, Kyoto, Japan (Ringgold ID: RIN65828)
,
Masataka Ikeda
5   Department of Gastroenterological Surgery, Division of Lower Gastrointestinal Surgery, Hyogo Medical University, Nishinomiya, Japan (Ringgold ID: RIN12818)
,
Shinichiro Shinzaki
1   Department of Gastroenterology, Hyogo Medical University, Nishinomiya, Japan (Ringgold ID: RIN12818)
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Background ‘Leaky gut’, caused by increased mucosal permeability, plays a pivotal role in various diseases. However, few methods are available to evaluate intestinal mucosal permeability in the living human body. We established a novel method for evaluation of mucosal permeability using indigo carmine (IC). Methods Subjects undergoing colonic endoscopy for screening of colon polyps or evaluation of ulcerative colitis (UC) severity were enrolled. IC was endoscopically sprayed in the cecum, and blood samples were obtained before spraying and at 30 and 60 min after. The level of serum IC was analyzed by LC/MS equipped with a Nexera HPLC system. Results In both the control (subjects screened for colon polyps) and UC groups, all subjects showed their highest serum IC levels at 30 min after spraying. The serum IC level was significantly higher in UC patients than in the controls at both 30 min and 60 min after spraying. In the UC group, serum IC levels at both 30 min and 60 min were significantly higher in patients with a Mayo endoscopic subscore (MES) 1 at the cecum than in those with MES 0 in the same area. Conclusions Endoscopic spraying with IC is useful for evaluation of intestinal mucosal permeability.



Publikationsverlauf

Eingereicht: 22. Oktober 2024

Angenommen nach Revision: 19. August 2025

Accepted Manuscript online:
08. September 2025

© . The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).

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Bibliographical Record
Hirokazu Fukui, Shojiro Kikuchi, Noriyuki Ojima, Tomonori Yokoyama, Masataka Ikeda, Shinichiro Shinzaki. Endoscopic indigo carmine spraying for evaluation of intestinal mucosal permeability: A prospective pilot study. Endosc Int Open ; 0: a26977599.
DOI: 10.1055/a-2697-7599