Polyphenols, Antioxidants, and Wound Healing of Lecythis pisonis Seed Coats

Elisângela Flávia Pimentel; Bruno Gomes de Oliveira; Ana Claudia Hertel Pereira; Mariana Moreira Figueira; Danielle Braga Portes; Rodrigo Scherer; Fabiana Gomes Ruas; Wanderson Romão; Márcio Fronza; Denise Coutinho Endringer

doi:10.1055/a-2212-0262

Planta Medica, Inhaltsverzeichnis

Planta Med 2024; 90(03): 243-251
DOI: 10.1055/a-2212-0262

Biological and Pharmacological Activity

Original Papers

Polyphenols, Antioxidants, and Wound Healing of Lecythis pisonis Seed Coats

Elisângela Flávia Pimentel

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Bruno Gomes de Oliveira

³Federal Institute of Espírito Santo, Vila Velha, Brazil

,

Ana Claudia Hertel Pereira

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Mariana Moreira Figueira

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Danielle Braga Portes

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Rodrigo Scherer

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Fabiana Gomes Ruas

²Capixaba Institute for Research, Technical Assistance, and Rural Extension, Vitoria, Brazil

,

Wanderson Romão

³Federal Institute of Espírito Santo, Vila Velha, Brazil

,

Márcio Fronza

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

,

Denise Coutinho Endringer

¹Pharmaceutical Science Graduate Program, Vila Velha University, Vila Velha, ES, Brazil

› Institutsangaben

Abstract

To better use the Lecythis pisonis Cambess. biomass, this study investigates whether Sapucaia seed coats present wound healing properties. We analyzed the antibacterial, antioxidant, and wound healing-promoting potentials, plus cytotoxicity and stimulation of vascular endothelial growth factor-A. The chemical composition was analyzed by positive ion mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. A total of 19 compounds were identified, such as proanthocyanidin A1, procyanidins A1, B2, and C1, epigallocatechin, and kaempferol (p-coumaroyl) glycoside. Potent antioxidant strength/index was verified for 2,2-diphenyl-1-picrylhydrazyl radical (IC₅₀ = 0.99 µg/mL) and ferric reducing antioxidant power (IC₅₀ = 1.09 µg/mL). The extract did not present cytotoxicity and promoted significant cell migration and/or proliferation of fibroblasts (p < 0.05). Vascular endothelial growth factor-A was stimulated dose-dependently at 6 µg/mL (167.13 ± 8.30 pg/mL), 12.5 µg/mL (210.3 ± 14.2 pg/mL), and 25 µg/mL (411.6 ± 29.4 pg/mL). Platelet-derived growth factor (PDGF) (0.002 µg/mL) was stimulated at 215.98 pg/mL. Staphylococcus aureus was susceptible to the extract, with a minimum inhibitory concentration of 31.25 µg/mL. The identified compounds benefit the antioxidant activity, promoting hemostasis for the wound healing process, indicating that this extract has the potential for use in dermatological cosmetics.

Key words

Lecythis pisonis Cambess - Lecythidaceae - chemical analysis - wound healing

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Referenzen

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