Network Pharmacology and Mechanism Studies of the Protective Effect of Ginseng against Alzheimerʼs Disease Based on Aβ Pathogenesis

Jinman Liu; Wenqian Yu; Cuiru Ma; Tianyao Li; Yong Liang; Shijie Su; Guangcheng Zhong; Zhouyuan Xie; Qiqing Wu; Jiaxin Chen; Qi Wang

doi:10.1055/a-2014-6061

Planta Medica, Table of Contents

Planta Med 2023; 89(10): 990-1000
DOI: 10.1055/a-2014-6061

Biological and Pharmacological Activity

Original Papers

Network Pharmacology and Mechanism Studies of the Protective Effect of Ginseng against Alzheimerʼs Disease Based on Aβ Pathogenesis

Authors

Jinman Liu

¹Affiliated Jiangmen TCM Hospital of Jiʼnan University, Jiangmen, China
Wenqian Yu

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Cuiru Ma

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Tianyao Li

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Yong Liang

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Shijie Su

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Guangcheng Zhong

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Zhouyuan Xie

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Qiqing Wu

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Jiaxin Chen

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China
Qi Wang

²Science and Technology Innovation Center, Guangzhou University of Chinese Medicine, Guangzhou, China

Abstract

Alzheimerʼs disease (AD) is a critical neurodegenerative disease that manifests as progressive intellectual decline and is pathologically characterized by a progressive loss of neurons in the brain. Despite extensive research on this topic, the pathogenesis of AD is not fully understood, while the beta-amyloid (Aβ) hypothesis remains the dominant one and only a few symptomatic drugs are approved for the treatment of AD. Ginseng has been widely reported as an effective herbal medicine for the treatment of neurodegenerative diseases such as dementia. Therefore, we explore the protective effects of ginseng in AD by a network pharmacological approach based on the pathogenesis of Aβ. Twenty-one major ginsenosides are screened based on ultraperformance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) data. Among them, MAPK8, MAPK9, BACE1, FLT1, CDK2, and CCR5 are the core targets. By molecular docking and validation with the in vitro cell model APPswe-SH-SY5Y, we find that ginsenosides Rg3 and Ro have good neuroprotective effects and can reduce the expression of Aβ _1 – 42 in APPswe-SH-SY5Y. Finally, through RT-qPCR experiment, we find that ginsenoside Rg3 targeted MAPK8, FLT1, and CCR5, while ginsenoside Ro targeted MAPK8, MAPK9, FLT1, and CCR5 for its potential anti-AD efficacy.

Key words

Ginseng - Araliaceae - Alzheimerʼs disease - beta-amyloid - network pharmacology - targets

Full Text

References

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Supplementary Material

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