UPLC/Q-TOF-MS-based Metabolomics Study of the Antiosteoporosis Effects of Vaccarin in Ovariectomized Mice

Fangming Song; Tianyu Xie; Xi Liu; Bonnie Chin; Xiaoting Luo; Shijie Liao; Wenyu Feng; Mingwei He; Nenggan Huang; Zhiheng Su; Yun Liu; Abu·Moro

doi:10.1055/a-1942-5428

Planta Medica, Inhaltsverzeichnis

Planta Med 2023; 89(02): 218-230
DOI: 10.1055/a-1942-5428

Natural Product Chemistry & Analytical Studies

Original Papers

UPLC/Q-TOF-MS-based Metabolomics Study of the Antiosteoporosis Effects of Vaccarin in Ovariectomized Mice

Fangming Song^*

¹Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China

,

Tianyu Xie^*

²Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

,

Xi Liu^*

³College of Chemistry and Chemical Engineering, Xiamen University, Nanning City, China

,

Bonnie Chin

⁴School of Postgraduate Studies, International Medical University, Kuala Lumpur, Malaysia

,

Xiaoting Luo

⁵Department of Pharmacy, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

,

Shijie Liao

¹Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China

,

Abu·Moro,

Wenyu Feng

²Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

,

Mingwei He

²Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

,

Nenggan Huang

²Department of Traumatic Orthopaedic, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

,

Zhiheng Su

⁷Pharmaceutical College, Guangxi Medical University, Nanning City, China

,

Yun Liu

⁶Department of Spine and Bone Diseases, the First Affiliated Hospital of Guangxi Medical University, Nanning City, China

¹Research Centre of Regenerative Medicine, Guangxi Medical University, Nanning City, China

› Institutsangaben

Abstract

Osteoporosis is a systemic and metabolic bone disease that usually occurs in postmenopausal women, which mainly manifests as bone loss and increased bone fragility that both facilitate fracture. However, few drugs for osteoporosis have shown good efficacy and limited side effects. Vaccarin has demonstrated its antiosteoporosis effects by inhibiting the formation and osteolytic activities of osteoclasts in our previous investigation. In this study, multivariate statistical analysis and ultrahigh-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry were used to analyze the serum metabolites of ovariectomized mice treated with or without vaccarin. As a result, 9 serum metabolites were identified as biomarkers. The metabolic levels of 3 crucial biomarkers, namely, lysophosphatidylcholine [22 : 6, (4Z, 7Z, 10Z, 13Z, 16Z, 19Z)], 1-linoleoylglycerophosphocholine and 1-palmitoyl-Sn-glycero-3-phosphocholine, that were correlated with glycerophospholipid metabolism increased and then decreased significantly after vaccarin treatment. Molecular docking analysis and osteoclasts differentiation experiment further revealed that vaccarin may bind with phospholipase A2 and downregulated its activity to reduce the osteoclastogenesis. Therefore, the occurrence of osteoporosis is closely related with glycerophospholipid metabolism disorders, and vaccarin exerts antiosteoporosis effects by reducing the levels of glycerophospholipid metabolites.

Key words

postmenopausal osteoporosis - glycerophospholipids metabolism - phospholipase A2 - vaccarin - Vaccaria segetalis - Caryophyllaceae

Volltext

Referenzen

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