Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-κB/NLRP3 Pathway to Alleviate LPS-induced Acute Lung
          Injury

Fanglei Zhang; Chang Ke; Zhongshi Zhou; Kang Xu; Yan Wang; Yanju Liu; Jiyuan Tu

doi:10.1055/a-1878-5704

Planta Medica, Inhaltsverzeichnis

Planta Med 2023; 89(05): 493-507
DOI: 10.1055/a-1878-5704

Biological and Pharmacological Activity

Original Papers

Scutellaria baicalensis Pith-decayed Root Inhibits Macrophage-related Inflammation Through the NF-κB/NLRP3 Pathway to Alleviate LPS-induced Acute Lung Injury

Fanglei Zhang‡

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Chang Ke‡

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Zhongshi Zhou

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Kang Xu

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

²Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Yan Wang

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Yanju Liu

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

²Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P. R. China

,

Jiyuan Tu

¹School of Pharmacy, Hubei University of Chinese Medicine, Wuhan, P. R. China

²Hubei Research Center of Chinese Materia Medica Processing Engineering and Technology, Hubei University of Chinese Medicine, Wuhan, P. R. China

› Institutsangaben

Abstract

Acute lung injury (ALI) is one of the representative “lung heat syndromes” in traditional Chinese medicine (TCM). Scutellaria baicalensis is an herbal medicine used in TCM for treating lung diseases, due to its remarkable anti-inflammatory and antiviral effects. When used in TCM, S. baicalensis root is divided into two categories: S. baicalensis pith-not-decayed root (SN) and S. baicalensis pith-decayed root (SD). Compared to SN, SD has a better effect on lung diseases. We constructed a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model to study the pharmacodynamic mechanism of SD. The ethanolic extract of Scutellaria baicalensis pith-decayed root (EESD) significantly affected LPS-induced ALI by reducing alveolar interstitial thickening, pulmonary edema, and other pathological symptoms, decreasing the infiltration of inflammatory cells, especially macrophages, and inhibiting IL-1β, TNF-α, and IL-6 transcription and translation. Furthermore, in the THP-1 macrophage model induced by LPS, EESD inhibited the expression of phosphorylated nuclear factor inhibitory protein alpha (p-IκBα), phosphorylated nuclear factor-κB P65 (p-p65), cleaved-caspase-1, cleaved-IL-1β protein, and the release of inflammatory factors in the NF-κB/NLRP3 pathway, inhibiting macrophage function. In vivo experiments yielded similar results. Therefore, the present study clarified the potential of EESD in the treatment of ALI and revealed its potential pharmacodynamic mechanism by inhibiting the NF-κB/NLRP3 inflammasome pathway and suppressing the pro-inflammatory phenotype activation of lung tissue macrophages.

Key words

Scutellaria baicalensis - acute lung injury - macrophages - NF-κB - NLRP3

Volltext

Referenzen

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