Study on the Action Mechanism of Dkk-1, TGF-β1 and TNF-α Expression Levels in Dupuytren’s Contracture

Abstract

Background The biological mechanism of Dupuytren’s contracture needs to be further studied in order to minimize postoperative recurrence and provide a pathological basis for the development of new therapeutic targets.

Methods HE staining, immunohistochemistry, PCR and western blotting were performed in pathological palmar aponeurosis specimens and normal palmar aponeurosis tissues for comparative study.

Results (1) TNF-α expression was up-regulated: TNF-α mRNA was more highly expressed in the pathological tissues of DD patients than in the CT group, P < 0.05, and the difference between the two groups was statistically significant; (2) Dkk-1 expression was down-regulated: Dkk-1 mRNA was lower expressed in the pathological tissues of DD patients than in the CT group, P < 0.05, and the difference between the two groups was statistically significant; (3) TGF-β1 expression was up-regulated: TGF-β1 mRNA was higher expressed in the pathological tissues of DD patients than in the CT group, P < 0.05, and the difference between the two groups was statistically significant; (4) Pearson correlation analysis suggested that TNF-α expression was positively correlated with TGF-β1 expression, TNF-α expression was negatively correlated with DKK-1 expression, and TGF-β1 expression was negatively correlated with DKK-1 expression.

Conclusion TNF-α, DKK-1 and TGF-β1 may play a role in the pathogenesis of palmar aponeurosis contracture, and there is a relationship between them. The study of the relationship between the three and their related signaling pathways provides a therapeutic target and a basis for the prevention and early treatment of palmar aponeurotic contracture.

Zusammenfassung

Hintergrund Der biologische Mechanismus der Dupuytren-Kontraktur muss weiter untersucht werden, um ein postoperatives Rezidiv zu minimieren und eine pathologische Grundlage für die Entwicklung neuer therapeutischer Ziele zu liefern.

Methoden HE-Färbung, Immunhistochemie, PCR und Western-Blotting wurden in pathologischen Palmaraponeurose-Proben und normalem Palmaraponeurose-Gewebe für vergleichende Untersuchungen durchgeführt.

Ergebnisse (1) TNF-α-Expression war hochreguliert: TNF-α-mRNA wurde in pathologischen Geweben von DD-Patienten stärker exprimiert als in der CT-Gruppe, P < 0,05, und der Unterschied zwischen den beiden Gruppen war statistisch signifikant; (2) Dkk-1-Expression war herunterreguliert: Dkk-1-mRNA wurde in pathologischen Geweben von DD-Patienten niedriger exprimiert als in der CT-Gruppe, P < 0,05, und der Unterschied zwischen den beiden Gruppen war statistisch signifikant; (3) TGF-β1-Expression war hochreguliert: TGF-β1-mRNA wurde in pathologischen Geweben von DD-Patienten stärker exprimiert als in der CT-Gruppe, P < 0,05, und der Unterschied zwischen den beiden Gruppen war statistisch signifikant; (4) Die Pearson-Korrelationsanalyse legte nahe, dass die TNF-α Expression positiv mit der TGF-β1-Expression korreliert war, die TNF-α-Expression negativ mit der DKK-1-Expression korrelierte und die TGF-β1-Expression negativ mit der DKK-1-Expression korrelierte.

Schlussfolgerung TNF-α, DKK-1 und TGF-β1 könnten eine Rolle bei der Pathogenese der Palmaraponeurose-Kontraktur spielen, und es besteht eine Beziehung zwischen ihnen. Die Untersuchung der Beziehung zwischen den drei und ihren verwandten Signalwegen bietet ein therapeutisches Ziel und eine Grundlage für die Vorbeugung und frühzeitige Behandlung der palmaraponeurotischen Kontraktur.

Key words

Dupuytren-Kontraktur - therapeutische Zielmoleküle

Schlüsselwörter

Dupuytren’s disease - TNF-α - DKK-1 - TGF-β1 - therapeutic targets

Publication History

Received: 14 September 2021

Accepted: 09 March 2022

Article published online:
13 April 2022

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