Toxicity of Hydrogen Sulfide on Rat Brain Neurons

Enayatollah Seydi; Zahra Irandoost; Mahmoud Ghazi Khansari; Parvaneh Naserzadeh; Farahnaz Tanbakosazan; Jalal Pourahmad

doi:10.1055/a-1750-8870

Drug Research, Inhaltsverzeichnis

Drug Res (Stuttg) 2022; 72(04): 197-202
DOI: 10.1055/a-1750-8870

Original Article

Toxicity of Hydrogen Sulfide on Rat Brain Neurons

Enayatollah Seydi

¹Department of Occupational Health and Safety Engineering, School of Health, Alborz University of Medical Sciences, Karaj, Iran

²Research Center for Health, Safety and Environment, Alborz University of Medical Sciences, Karaj, Iran

,

Zahra Irandoost

³Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Mahmoud Ghazi Khansari

⁴Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

,

Parvaneh Naserzadeh

³Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Farahnaz Tanbakosazan

³Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

Jalal Pourahmad

³Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

› Institutsangaben

Abstract

Hydrogen sulfide (H₂S) is a toxic compound known as a member of the gasotransmitter family. H₂S has the ability to inhibit the cytochrome c oxidase enzyme in the mitochondrial respiratory chain. Mitochondria play an important role in energy production and the brain needs energy for normal function. Mitochondrial dysfunction is associated with neurodegenerative diseases. This study investigated the mechanisms of cytotoxicity induced by H₂S in brain neurons. thioacetamide has been used to produce H₂S in water solutions. The results of the study showed that thioacetamide at concentrations of 116, 232 and 464 µg/ml was able to increase the level of reactive oxygen species (ROS), collapse in mitochondrial membrane potential (MMP), damage to the lysosomal membrane, increase in the level of oxidized glutathione (GSSG) and decrease in the level of reduced glutathione (GSH) in brain neurons. The results of the study suggested that H₂S causes damage to mitochondria and lysosomes in brain neurons that could be associated with neurodegenerative diseases.

Key words

Neurotoxicity - Hydrogen Sulfide - Mitochondria, Lysosomes - Thioacetamide

Volltext

Referenzen

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