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Drug Res (Stuttg) 2022; 72(04): 197-202
DOI: 10.1055/a-1750-8870
Original Article

Toxicity of Hydrogen Sulfide on Rat Brain Neurons

Enayatollah Seydi
1   Department of Occupational Health and Safety Engineering, School of Health, Alborz University of Medical Sciences, Karaj, Iran
2   Research Center for Health, Safety and Environment, Alborz University of Medical Sciences, Karaj, Iran
,
Zahra Irandoost
3   Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Mahmoud Ghazi Khansari
4   Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
,
Parvaneh Naserzadeh
3   Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Farahnaz Tanbakosazan
3   Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
,
Jalal Pourahmad
3   Department of Toxicology and Pharmacology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Abstract

Hydrogen sulfide (H2S) is a toxic compound known as a member of the gasotransmitter family. H2S has the ability to inhibit the cytochrome c oxidase enzyme in the mitochondrial respiratory chain. Mitochondria play an important role in energy production and the brain needs energy for normal function. Mitochondrial dysfunction is associated with neurodegenerative diseases. This study investigated the mechanisms of cytotoxicity induced by H2S in brain neurons. thioacetamide has been used to produce H2S in water solutions. The results of the study showed that thioacetamide at concentrations of 116, 232 and 464 µg/ml was able to increase the level of reactive oxygen species (ROS), collapse in mitochondrial membrane potential (MMP), damage to the lysosomal membrane, increase in the level of oxidized glutathione (GSSG) and decrease in the level of reduced glutathione (GSH) in brain neurons. The results of the study suggested that H2S causes damage to mitochondria and lysosomes in brain neurons that could be associated with neurodegenerative diseases.

Key words

Neurotoxicity - Hydrogen Sulfide - Mitochondria, Lysosomes - Thioacetamide


Publication History

Received: 25 December 2021

Accepted: 24 January 2022

Article published online:
17 February 2022

© 2022. Thieme. All rights reserved.

Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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