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Drug Res (Stuttg) 2022; 72(01): 47-52
DOI: 10.1055/a-1625-3121
Original Article

Quantification of a New Antifungal Drug Based on 1,3,4-Thiadiazole by HPLC-ESI/MS: Method Development, Validation and Pharmacokinetic Application

Nadezhda Yur'evna Sipkina 
1   St. Petersburg State Chemical Pharmaceutical University, St. Petersburg, Russia
,
Denis Igorevich Sipkin
2   Scheltec AG, Moscow, Russia
,
Igor Pavlovich Yakovlev
1   St. Petersburg State Chemical Pharmaceutical University, St. Petersburg, Russia
› Author Affiliations Funding This work was supported by the Ministry of Education of Russian Federation [government number 115080740020] and was made in Research Equipment Sharing Center “Analytical Center of St. Petersburg State Chemical Pharmaceutical University” [N 075–15–2021–685 26/07/2021].
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Abstract

The high sensitive HPLC-ESI/MS method for quantitative determination of a new antifungal drug – 2-[(1Z)-1-(3,5-diphenyl-1,3,4-thiadiazol-2(3Н)-ylidene)methyl]-3,5-diphenyl-1,3,4-thiadiazol-3-ium chloride (TDZ) – was developed and fully validated. TDZ was separated from plasma and urine samples by acetonitrile deproteinization and extraction without time-consuming sample preparation. The reversed-phase high-performance liquid chromatography on Kromasil 100–3.5 C8 column of TDZ in isocratic elution mode using 0.03% trifluoroacetic acid : acetonitrile (65:35, v/v) at a flow rate of 0.2 mL min−1 was performed. Determination of TDZ was carried out by a positive electrospray ionization in a selected ion monitoring mode for [M+]=489 m/z. The method of absolute calibration was used for quantification of TDZ in two concentrations ranges: 100–2500 pg mL−1 and 2500–30 000 pg mL−1. The established method showed a good linearity (R=0.999 for both ranges), the limits of determination and quantification were 50 and 100 pg mL−1, respectively. The Intra- and Inter-day precision values were measured by t-Distribution and Fisher′s Exact Test and were in accordance with the regulatory guidance. Low matrix effects and good recovery were found for TDZ. The present method was successfully applied to determine the pharmacokinetic parameters of TDZ by means of intravenous and oral administrations to rats at 5.0 mg kg−1 and 10.0 mg kg−1, respectively.

Key words

thiadiazoles - fungicides - liquid chromatography - mass-spectrometry - pharmacokinetics


Publication History

Received: 13 June 2021

Accepted: 30 August 2021

Article published online:
17 September 2021

© 2021. Thieme. All rights reserved.

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