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Planta Med 2021; 87(12/13): 1089-1100
DOI: 10.1055/a-1554-2733
Natural Product Chemistry and Analytical Studies
Original Papers

Cytotoxic, Antioxidant, and Antidiabetic Activities versus UPLC-ESI-QTOF-MS Chemical-Profile Analysis of Ipomoea aquatica Fractions[ # ]

Mahmoud Hefny Gad
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
2   Medicinal and Aromatic Plants Research Department, Horticulture Institute, Agricultural Research Center, Dokki, Giza, Egypt
,
Kristiaan Demeyer
3   Experimental In Vitro Toxicology and Dermato-Cosmetology, Vrije Universiteit Brussel (VUB), Brussels, Belgium
,
Yvan Vander Heyden
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
,
Debby Mangelings
1   Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium
› InstitutsangabenGefördert durch: Fonds Wetenschappelijk Onderzoek G033816N
Gefördert durch: Mundus MEDASTAR (Mediterranean Area for Science, Technology and Research) Mundus MEDASTAR/Grant agreement number (2011-4051/002-001-EMA2)
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Abstract

Ipomoea aquatica is a common green leafy vegetable that has numerous uses in traditional medicine. This study focused on the determination of the cytotoxic, antiradical, and antidiabetic properties of various fractions of the I. aquatica methanolic extract, as well as on the tentative identification of some bioactive compounds in the same fractions. The cytotoxicity was determined by the brine shrimp lethal test. The antioxidant activities of the I. aquatica fractions were investigated through 3 assays. The antidiabetic activity (in vitro) was measured by α-glucosidase and α-amylase inhibition assays. Phytochemical qualitative analyses demonstrated the presence of alkaloids, terpenoids, phenols, and flavonoids in the ethyl acetate-methanol and methanol fractions. The total phenolic and total flavonoid contents were found to be highest in the ethyl acetate-MeOH fractions. The evaluation of the cytotoxicity showed that the hexane-dichloromethane fraction is the most toxic, while the others are moderately toxic. The antioxidant activity assays showed that the ethyl acetate-MeOH fractions are the most potent, while the α-glucosidase and α-amylase assays revealed that the hexane-dichloromethane fraction might contain a potent antidiabetic agent. Some bioactive substances in the MeOH fractions, such as salicylic acid glucoside, 1-O-sinapoyl-β-D-glucose derivative, and dihydroferulic acid derivative, were tentatively identified. To the best of our knowledge, this is the first report to detect and identify these compounds in this species. Based on the results of this study, it may be concluded that I. aquatica is a potent antioxidant agent and could be a good candidate as a natural antioxidant in food and therapeutics.

Key words

Ipomoea aquatica - Convolvulaceae - chemical-profile analysis - cytotoxic activity - antioxidant activity - antidiabetic activity

# Dedicated to Professor Arnold Vlietinck on the occasion of his 80th birthday.




Publikationsverlauf

Eingereicht: 31. Januar 2021

Angenommen nach Revision: 09. Juli 2021

Artikel online veröffentlicht:
06. August 2021

© 2021. Thieme. All rights reserved.

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