The Reassessed Impact of Nicotine against Neurotoxicity in Mesencephalic Dopaminergic Cell Cultures and Neuroblastoma N18TG2 Cells

Marcin Delijewski; Khaled Radad; Christopher Krewenka; Barbara Kranner; Rudolf Moldzio

doi:10.1055/a-1527-1390

Planta Medica, Inhaltsverzeichnis

Planta Med 2022; 88(07): 548-558
DOI: 10.1055/a-1527-1390

Biological and Pharmacological Activity

Original Papers

The Reassessed Impact of Nicotine against Neurotoxicity in Mesencephalic Dopaminergic Cell Cultures and Neuroblastoma N18TG2 Cells

Marcin Delijewski

¹Department of Pharmacology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, Katowice, Poland

,

Khaled Radad

²Department of Pathology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt

,

Christopher Krewenka

³Institute of Medical Biochemistry, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

,

Barbara Kranner

³Institute of Medical Biochemistry, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

,

Rudolf Moldzio

³Institute of Medical Biochemistry, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria

› Institutsangaben

Abstract

Neuroprotective effects of nicotine are still under debate, so further studies on its effectiveness against Parkinsonʼs disease are required. In our present study, we used primary dopaminergic cell cultures and N18TG2 neuroblastoma cells to investigate the effect of nicotine and its neuroprotective potential against rotenone toxicity. Nicotine protected dopaminergic (tyrosine hydroxylase immunoreactive) neurons against rotenone. This effect was not nAChR receptor-dependent. Moreover, the alkaloid at a concentration of 5 µM caused an increase in neurite length, and at a concentration of 500 µM, it caused an increase in neurite count in dopaminergic cells exposed to rotenone. Nicotine alone was not toxic in either cell culture model, while the highest tested concentration of nicotine (500 µM) caused growth inhibition of N18TG2 neuroblastoma cells. Nicotine alone increased the level of glutathione in both cell cultures and also in rotenone-treated neuroblastoma cells. The obtained results may be helpful to explain the potential neuroprotective action of nicotine on neural cell cultures.

Key words

nicotine - Nicotiana tabacum - Solanaceae - dopaminergic neurones - neuroblastoma - rotenone

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Referenzen

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