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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(02): e56-e61
DOI: 10.1055/a-1492-3585
Original Paper

Pachypodostyflavone, a New 3-methoxy Flavone and Other Constituents with Antifilarial Activities from the Stem Bark of Duguetia staudtii

Alexis Sylvain Wafo Mbobda
1   Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé, Cameroon
8   Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon
,
Alain Wembe Ngouonpe
2   Department of Chemistry, Faculty of Science, University of Buea, Buea, Cameroon
,
Gervais Mouthé Happi
3   Department of Chemistry, Higher Teacher Training College of Bambili, University of Bamenda, Bambili, Cameroon
,
Bel Youssouf G. Mountessou
1   Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé, Cameroon
,
Elvis Monya
4   Biotechnology Unit, Faculty of Science, University of Buea, South West Region, Cameroon
,
Jean-Bosco Jouda
5   Chemical Engineering and Mineral Industries School, EGCIM, University of Ngaoundere, Ngaoundere, Cameroon
,
Billy Tchegnitegni Toussie
6   Department of Chemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
,
Bruno Ndjakou Lenta
1   Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé, Cameroon
,
Norbert Sewald
7   Organic and Bioorganic Chemistry, Faculty of Chemistry, Bielefeld University, Bielefeld, Germany
,
Simeon Fogue Kouam
1   Department of Chemistry, Higher Teacher Training College, University of Yaoundé I, Yaoundé, Cameroon
,
Jean Claude Tchouankeu
8   Department of Organic Chemistry, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, Cameroon
› Author Affiliations Funding A.S.W.M. is grateful to the German Academic Exchange Service (DAAD) for the financial support to the Yaoundé-Bielefeld Graduate School of Natural Products with Antiparasite and Antibacterial activities (YaBiNaPA), project N ° 57316173, and they wish also to express their heartfelt gratitude to The World Academy of Science (TWAS) (grant N ° FR3240303654), and the Alexander von Humboldt Foundation for the equipment support to our laboratory at the Higher Teacher Training College, University of Yaoundé I.
› Further Information
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Abstract

A new flavone derivative named pachypodostyflavone (1), along with 8 known compounds (2–9) and a mixture of β-sitosterol and stigmasterol were isolated from the stem bark of Duguetia staudtii (Annonaceae), based on a bioassay-guided fractionation. Their structures were determined using high-resolution mass spectrometry and NMR spectroscopic data, as well as by comparison with the literature values of their analogs. Selected isolated compounds were evaluated for their in vitro antifilaricidal activities on Onchocerca ochengi microfilariae and adult worms. Inhibition of motility was evaluated spectroscopically on microfilaria and adult male worms. Viability was determined on adult female worms by the MTT/ Formazan assay. Auranofin at 10 µM and 2% DMSO were used as positive and negative controls, respectively. Compounds 1 and 7 showed potent anti-onchocerca activities with 100% activity at 250 µg/mL on both O. ochengi adult male and female worms, while compound 5 displayed 100% activity at 30 µg/mL.

Key words

Duguetia staudtii - Annonaceae - bio-guided fractionation - flavone - pachypodostyflavone - antifilarial activity - Onchocerca ochengi microfilariae

Supplementary Material



Publication History

Received: 20 January 2021

Accepted: 19 April 2021

Article published online:
14 July 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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