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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(02): e62-e68
DOI: 10.1055/a-1484-9750
Original Papers

Cytokinin Mediated Increased In Vitro Production of Secondary Metabolites with Special Reference to Solasodine in Solanum erianthum

Jeeta Sarkar
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany (DST-FIST and UGC-DRS Funded), Visva-Bharati (A Central University), Santiniketan, West Bengal, India
,
Nirmalya Banerjee
Cytogenetics and Plant Biotechnology Laboratory, Department of Botany (DST-FIST and UGC-DRS Funded), Visva-Bharati (A Central University), Santiniketan, West Bengal, India
› Author Affiliations Funding: The first author is grateful to the University Grants Commission, Govt. of India, for financial support in the form of a UGC-BSR fellowship [No. F.25-1/2013-14(BSR)/7-220/2009(BSR)].
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Abstract

Steroid alkaloid solasodine is a nitrogen analogue of diosgenin and has great importance in the production of steroidal medicines. Solanum erianthum D. Don (Solanaceae) is a good source of solasodine. The aim of this study was to evaluate the effect of different cytokinins on the production of secondary metabolites, especially solasodine in the in vitro culture of S. erianthum. For solasodine estimation, field-grown plant parts and in vitro tissues were extracted thrice and subjected to high-performance liquid Chromatography. Quantitative analysis of different secondary metabolites showed that the amount was higher in the in vitro regenerated plantlets compared to callus and field-grown plants. The present study critically evaluates the effect of the type of cytokinin used in the culture medium on solasodine accumulation in regenerated plants. The highest solasodine content (46.78±3.23 mg g-1) was recorded in leaf extracts of the in vitro grown plantlets in the presence of 6-γ,γ-dimethylallylamino purine in the culture medium and the content was 3.8-fold higher compared to the mother plant.

Key words

Solanum erianthum - Solanaceae - cytokinin - solasodine

Supplementary Material



Publication History

Received: 25 November 2020
Received: 04 March 2021

Accepted: 12 April 2021

Article published online:
13 July 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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