Abstract
Repeated exposure to a high-fat meal triggers inflammation and oxidative stress,
contributing to the onset of cardiometabolic diseases. Regular exercise prevents
cardiometabolic diseases and a prior bout of acute endurance exercise can
counteract the detrimental cardiovascular effects of a subsequent high-fat meal.
Circulating microRNAs (ci-miRs) are potential mediators of these vascular
effects through regulation of gene expression at the posttranscriptional level.
Therefore, we investigated the expression of ci-miRs related to vascular
function (miR-21, miR-92a, miR-126, miR-146a, miR-150, miR-155, miR-181b,
miR-221, miR-222) in plasma from healthy, recreationally to highly active,
Caucasian adult men after a high-fat meal with (EX) and without (CON) a
preceding bout of cycling exercise. Ci-miR-155 was the only ci-miR for which
there was a significant interaction effect of high-fat meal and exercise
(p=0.050). Ci-miR-155 significantly increased in the CON group at two (p=0.007)
and four hours (p=0.010) after the high-fat meal test, whereas it significantly
increased in the EX group only four hours after the meal (p=0.0004). There were
significant main effects of the high-fat meal on ci-miR-21 (p=0.01), ci-miR-126
(p=0.02), ci-miR-146a (p=0.02), ci-miR-181b (p=0.02), and ci-miR-221 (p=0.008).
Collectively, our results suggest that prior exercise does not prevent
high-fat meal-induced increases in vascular-related ci-miRs.
Key words
microRNA - exercise - postprandial lipemia - vascular
