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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(02): e43-e55
DOI: 10.1055/a-1471-8947
Original Papers

Anti-inflammatory and Anti-arthritic Activities of Aqueous Extract and Flavonoids from Tripodanthus acutifolius Leaves in Mice Paw Oedema

Luis Apaza Ticona 
1   Department of Organic Chemistry, Faculty of Sciences, University Autónoma of Madrid, Madrid, Spain
2   Department of Pharmacology, Pharmacognosy and Botany, Faculty of Pharmacy, University Complutense of Madrid, Madrid, Spain
,
Andreea Madalina Serban
3   Maria Sklodowska Curie University Hospital for Children, Constantin Brancoveanu Boulevard, Bucharest, Romania
,
Daly Apaza Ticona
4   Clin & Gen SRL Laboratory, La Paz, Bolivia
,
Karla Slowing
2   Department of Pharmacology, Pharmacognosy and Botany, Faculty of Pharmacy, University Complutense of Madrid, Madrid, Spain
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Abstract

Tripodanthus acutifolius, commonly known as Jamillo, is an herbal remedy used in traditional Andean medicine to treat joint problems, such as sprains, dislocations, and rheumatic pain. This study aimed to evaluate the in vitro and in vivo anti-inflammatory and anti-arthritic activity of the aqueous extract and isolated compounds of T. acutifolius. A bioguided phytochemical analysis based on NMR/MS was performed to identify the compounds of the aqueous extract from T. acutifolius. The anti-inflammatory and anti-arthritic activity were evaluated by measuring inflammatory parameters (TNF-α, C-reactive protein, and fibrinogen) in murine models. The chemical structure determination led to the identification of four flavonoids: (E)-2’,4’-dihydroxy-6’-methoxy-chalcone (1), 6,2’,4’-trimethoxyflavone (2), 5,3’,4’-trihydroxy-6,7,8-trimethoxyflavone (3), and 5,4’-dihydroxy-6,7,8-trimethoxyflavone (4). All compounds inhibited the production of TNF-α in the RAW 264.7 cell line, with IC50 values of 0.78, 1.43, 5.73, and 9.71 μM, respectively. In addition, all flavonoids decreased the levels of TNF-α, C-reactive protein, and fibrinogen at a concentration of 5 mg/kg in murine models. Our research shows that these compounds isolated from T. acutifolius have anti-inflammatory and anti-arthritic properties, providing scientific evidence for the traditional use of this plant species.

Key words

Loranthaceae - Tripodanthus acutifolius - flavonoids - anti-inflammatory - anti-arthritic - TNF-α

Supplementary Material



Publication History

Received: 13 February 2021
Received: 21 March 2021

Accepted: 30 March 2021

Article published online:
27 May 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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