CC BY-NC-ND 4.0 · Horm Metab Res 2021; 53(05): 326-334
DOI: 10.1055/a-1440-0278
Endocrine Care

Adrenal Hormone Interactions and Metabolism: A Single Sample Multi-Omics Approach

1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
2  Department of Medicine III, Technische Universität Dresden, Dresden, Germany
3  German Institute of Human Nutrition Potsdam-Rehbruecke, Department of Experimental Diabetology, Nuthetal, Germany
4  German Center for Diabetes Research (DZD), München-Neuherberg, Germany
,
Deepika Watts
1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
,
2  Department of Medicine III, Technische Universität Dresden, Dresden, Germany
,
Paal William Wallace
1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
,
Annette Schürmann
3  German Institute of Human Nutrition Potsdam-Rehbruecke, Department of Experimental Diabetology, Nuthetal, Germany
4  German Center for Diabetes Research (DZD), München-Neuherberg, Germany
,
Stefan R. Bornstein
2  Department of Medicine III, Technische Universität Dresden, Dresden, Germany
,
Ben Wielockx
1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
,
Graeme Eisenhofer
1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
2  Department of Medicine III, Technische Universität Dresden, Dresden, Germany
,
Mirko Peitzsch
1  Institute of Clinical Chemistry and Laboratory Medicine, Technische Universität Dresden, Dresden, Germany
› Author Affiliations
Funding Deutsche Forschungsgemeinschaft (DFG) CRC/Transregio 205/1 (Project No. 314061271), INST 515/28–1 FUGG and German Ministry of Education and Research and the Brandenburg State 82DZD00302.

Abstract

The adrenal gland is important for many physiological and pathophysiological processes, but studies are often restricted by limited availability of sample material. Improved methods for sample preparation are needed to facilitate analyses of multiple classes of adrenal metabolites and macromolecules in a single sample. A procedure was developed for preparation of chromaffin cells, mouse adrenals, and human chromaffin tumors that allows for multi-omics analyses of different metabolites and preservation of native proteins. To evaluate the new procedure, aliquots of samples were also prepared using conventional procedures. Metabolites were analyzed by liquid-chromatography with mass spectrometry or electrochemical detection. Metabolite contents of chromaffin cells and tissues analyzed with the new procedure were similar or even higher than with conventional methods. Catecholamine contents were comparable between both procedures. The TCA cycle metabolites, cis-aconitate, isocitate, and α-ketoglutarate were detected at higher concentrations in cells, while in tumor tissue only isocitrate and potentially fumarate were measured at higher contents. In contrast, in a broad untargeted metabolomics approach, a methanol-based preparation procedure of adrenals led to a 1.3-fold higher number of detected metabolites. The established procedure also allows for simultaneous investigation of adrenal hormones and related enzyme activities as well as proteins within a single sample. This novel multi-omics approach not only minimizes the amount of sample required and overcomes problems associated with tissue heterogeneity, but also provides a more complete picture of adrenal function and intra-adrenal interactions than previously possible.

Supplementary Material



Publication History

Received: 11 January 2021

Accepted after revision: 11 March 2021

Publication Date:
26 April 2021 (online)

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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