Drug Res (Stuttg) 2021; 71(07): 379-387
DOI: 10.1055/a-1389-5499
Original Article

Lansoprazole a Proton Pump Inhibitor Prevents IBD by Reduction of Oxidative Stress and NO Levels in the Rat

Tejal Gandhi
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
,
Anish Sharma
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
,
Navdha Vyas
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
,
Parth Gupta
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
,
Mihir Parikh
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
,
Hital Shah
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat India
› Author Affiliations
Acknowledgment: No funding was received for this manuscript. This is an Anand Pharmacy College communication.

Abstract

The inflammatory disease’s increased prevalence leads to a major concern around the world. Still, there is a lack of effective and successful therapy in the reversal of Inflammatory Bowel Disease (IBD) symptoms. Whereas, reactive oxygen species (ROS) production and muddled defense capacity of antioxidants in IBD subjects reported several times. Many proton pump inhibitors have been reported previously for their anti-inflammatory effect. The present study is aimed to assess the ameliorative effect of lansoprazole in experimentally induced IBD in rats. Thirty-six female Sprague Dawley rats were divided equally into six groups based on their body weight. Lansoprazole (1, 5, and 10 mg/kg, p.o.) and 5-aminosalicylate (5-ASA, 100 mg/kg, p.o.) served as standard control respectively, given for 18 days once a day. On the 11th day of the study, colitis was induced by intrarectal instillation of 2, 4-Dinitrobenzene sulfonic acid (DNBS), and treatment was continued for the next 7 days. Administration of lansoprazole (at 5 and 10 mg/kg) significantly reduced DAI (Disease Activation Index) and CMDI (Colon Macroscopic Damage Index); which further justifies a reduction in colon inflammation grades, as well as histopathological changes, and reflected by the stalling of body weight. The anti-inflammatory effects were indicated by lowered MPO (myeloperoxidase) and SOD (superoxide dismutase) in colon tissue as well as restores colonic NO (nitric oxide) level. The study shows lansoprazole improved DAI and CMDI scores, reduction of neutrophil infiltration, and an improved antioxidant status indicating an anti-ulcerative effect in DNBS-induced experimental colitis that is comparable with 5-ASA treatment.



Publication History

Received: 06 December 2020

Accepted: 08 February 2021

Publication Date:
16 March 2021 (online)

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany