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DOI: 10.1055/a-1379-3249
DNA Barcoding of St. Johnʼs wort (Hypericum spp.) Growing Wild in North-Eastern Greece
Gefördert durch: European Regional Development Fund MIS 5002803 Gefördert durch: National Strategic Reference Framework (NSRF), Research Funding Programme of the Action RESEARCH – CREATE – INNOVATE AROMADISTIL – 95783
Abstract
Plants of the genus Hypericum, commonly known as “St. Johnʼs wort” (“spathohorto” or “valsamo” in Greek), have been used since antiquity for their therapeutic properties. Wild-harvested Hypericum plants are still popular today in herbal medicines, commercially exploited due to their bioactive compounds, hypericin and hyperforin, which have antidepressant, antimicrobial and antiviral activity. Species identification of commercial products is therefore important and DNA barcoding, a molecular method that uses small sequences of organismsʼ genome as barcodes, can be useful in this direction. In this study, we collected plants of the genus Hypericum that grow wild in North-Eastern Greece and explored the efficiency of matK, and trnH-psbA regions as DNA barcodes for their identification. We focused on 5 taxa, namely H. aucheri, H. montbretii, H. olympicum, H. perforatum subsp. perforatum, and H. thasium, the latter a rare Balkan endemic species collected for the first time from mainland Greece. matK (using the genus-specific primers designed herein), trnH-psbA, and their combination were effectively used for the identification of the 5 Hypericum taxa and the discrimination of different H. perforatum subsp. perforatum populations. These barcodes were also able to discriminate Greek populations of H. perforatum, H. aucheri, H. montbretii, and H. olympicum from populations of the same species growing in other countries.
Supporting Information
- Supporting Information
NJ tree for (a) matK, (b) rbcL, and (c) trnH-psbA sequences retrieved from GenBank based on K2P model of nucleotide substitution (Fig. 1S), matK and trnH-psbA sequence length (Fig. 2S), PCR products of (a) matK and (b) trnH-psbA as shown in agarose gel 1% (Fig. 3S), nucleotide sequence alignment of (a) matK, (b) trnH-psbA, and (c) matK + trnH-psbA of Hypericum taxa collected from NE Greece (Fig. 4S). Percentage of variable sites in matK, rbcL and trnH-psbA sequences retrieved from GenBank (Table 1S), percentage of PCR and sequence success (Table 2S), percentage of variable characters in matK and trnH-psbA sequences (Table 3S), K2P genetic distances in matK (Table 4S), trnH-psbA (Table 5S) and matK + trnH-psbA sequences (Table 6S), nucleotide primers used in this study (Table 7S).
Publikationsverlauf
Eingereicht: 10. Oktober 2020
Angenommen nach Revision: 28. Januar 2021
Artikel online veröffentlicht:
22. Februar 2021
© 2021. Thieme. All rights reserved.
Georg Thieme Verlag KG
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