Planta Med 2021; 87(08): 611-619
DOI: 10.1055/a-1351-1038
Biological and Pharmacological Activity
Original Papers

BNO 1095, a Standardized Dry Extract from the Fruits of Vitex agnus-castus, Impairs Angiogenesis-related Endothelial Cell Functions In Vitro

Iris Bischoff-Kont
1  Institute of Pharmaceutical Biology, Goethe University Frankfurt/Main, Germany
,
Laura Brabenec
1  Institute of Pharmaceutical Biology, Goethe University Frankfurt/Main, Germany
,
Rebecca Ingelfinger
1  Institute of Pharmaceutical Biology, Goethe University Frankfurt/Main, Germany
2  LOEWE Center Translational Biodiversity Genomics (TBG), Frankfurt/Main, Germany
,
Bernhard Nausch
3  Bionorica SE, Neumarkt, Germany
,
Robert Fürst
1  Institute of Pharmaceutical Biology, Goethe University Frankfurt/Main, Germany
2  LOEWE Center Translational Biodiversity Genomics (TBG), Frankfurt/Main, Germany
› Author Affiliations

Abstract

BNO 1095, a standardized dry extract from the fruits of Vitex agnus-castus, represents an approved herbal medicinal product for the treatment of premenstrual syndrome. Angiogenesis, the formation of new blood vessels from pre-existing capillaries, plays a major role in physiological situations, such as wound healing or tissue growth in female reproductive organs, but it is also of great importance in pathophysiological conditions such as chronic inflammatory diseases or cancer. Angiogenesis is a highly regulated multi-step process consisting of distinct key events that can be influenced pharmacologically. Few studies suggested anti-angiogenic actions of V. agnus-castus fruit extracts in in vivo and ex vivo models. Here, we provide for the first time profound in vitro data on BNO 1095-derived anti-angiogenic effects focusing on distinct angiogenesis-related endothelial cell functions that are inevitable for the process of new blood vessel formation. We found that V. agnus-castus extract significantly attenuated undirected and chemotactic migration of primary human endothelial cells. Moreover, the extract efficiently inhibited endothelial cell proliferation and reduced the formation of tube-like structures on Matrigel. Of note, the treatment of endothelial cell spheroids almost blocked endothelial sprouting in a 3D collagen gel. Our data present new and detailed insights into the anti-angiogenic actions of BNO 1095 and, therefore, suggest a novel scope of potential therapeutic applications of the extract for which these anti-angiogenic properties are required.

Supporting Information



Publication History

Received: 12 October 2020

Accepted after revision: 04 January 2021

Publication Date:
02 February 2021 (online)

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany