Planta Med 2021; 87(01/02): 148-159
DOI: 10.1055/a-1301-0183
Biological and Pharmacological Activities
Original Papers

Infraspecific Chemical Variability and Biological Activity of Casearia sylvestris from Different Brazilian Biomes

Paula Carolina Pires Bueno
1  Institute of Chemistry, Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil
,
Luis Francisco Salomé Abarca
2  Natural Products Laboratory, Institute of Biology, Leiden University, Leiden, The Netherlands
,
Naira Buzzo Anhesine
1  Institute of Chemistry, Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil
,
Maíra Silva Giffoni
1  Institute of Chemistry, Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil
,
Fabiola Manhas Verbi Pereira
1  Institute of Chemistry, Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil
3  National Institute of Alternative Technologies for Detection Toxicological Assessment and Removal of Micropollutants and Radioactive Substances (INCT-DATREM) Araraquara, São Paulo State, Brazil
,
Roseli Buzaneli Torres
4  Agronomic Institute of Campinas, Herbarium IAC, Campinas/SP, Brazil
,
Rayran Walter RamosSousa de
5  Department of Biophysics and Physiology, Laboratory of Experimental Cancerology, Federal University of Piauí, Teresina/PI, Brazil
,
Paulo Michel Pinheiro Ferreira
5  Department of Biophysics and Physiology, Laboratory of Experimental Cancerology, Federal University of Piauí, Teresina/PI, Brazil
,
Claudia Pessoa
6  Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza/CE, Brazil
,
Alberto José Cavalheiro
1  Institute of Chemistry, Department of Biochemistry and Organic Chemistry, São Paulo State University (UNESP), Araraquara/SP, Brazil
› Author Affiliations
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico 307328/2019-8
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico 465571/2014-0
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico 563311/2010-0
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2010/52327-5
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2011/21440-3
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2012/21921-4
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2013/07600-3
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/02738-0
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/50945-4
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/20295-3
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/18212-8
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2019/01102-8
Supported by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 88887136426/2017/00

Abstract

Casearia sylvestris is an outstanding representative of the Casearia genus. This representability comes from its distinctive chemical profile and pharmacological properties. This species is widespread from North to South America, occurring in all Brazilian biomes. Based on their morphology, 2 varieties are recognized: C. sylvestris var. sylvestris and C. sylvestris var. lingua. Despite the existence of data about their chemical composition, a deeper understanding of the specialized metabolism correlation and variation in respect to environmental factors and its repercussion over their biological activities was still pending. In this study, an UHPLC-DAD-based metabolomics approach was employed for the investigation of the chemical variation of 12 C. sylvestris populations sampled across 4 Brazilian biomes and ecotones. The correlation between infraspecific chemical variability and the cytotoxic and antioxidant activities was achieved by multivariate data analysis. The analyses showed that C. sylvestris var. lingua prevailed at Cerrado areas, and it was correlated with lower cytotoxic activity and high level of glycosylated flavonoids. Among them, narcissin and isorhamnetin-3-O-α-L-rhamnopyranosyl-(1 → 2)-α-L-arabinopyranoside showed good correlation with the antioxidant activity. Conversely, C. sylvestris var. sylvestris prevailed at the Atlantic Forest areas, and it was associated with high cytotoxic activity and high content of clerodane diterpenoids. Different casearins showed good correlation (R2  = 0.3 – 0.70) with the cytotoxic activity. These findings highlighted the great complexity among different C. sylvestris populations, their chemical profile, and the related biological activities. Consequently, it can certainly influence the medicinal properties, as well as the quality and efficacy, of C. sylvestris phytomedicines.

Supporting Information



Publication History

Received: 28 June 2020

Accepted after revision: 26 October 2020

Publication Date:
21 December 2020 (online)

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