Planta Med 2021; 87(03): 218-224
DOI: 10.1055/a-1279-0645
Biological and Pharmacological Activity
Original Papers

Antiproliferative Effects of Pterodon pubescens Extract and Isolated Diterpenes in HaCaT Cells

Rosanna Tarkany Basting
1  Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil
2  Postgraduate Program in Medical Sciences, Faculty of Medical Science (FCM), University of Campinas (Unicamp), Campinas, SP, Brazil
3  Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paulínia, SP, Brazil
4  São Leopoldo Mandic Institute and Research Center, Laboratory of Neuroimmune Interface of Pain Research, Campinas, SP, Brazil
,
Ilza Maria de Oliveira Sousa
1  Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil
2  Postgraduate Program in Medical Sciences, Faculty of Medical Science (FCM), University of Campinas (Unicamp), Campinas, SP, Brazil
3  Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paulínia, SP, Brazil
,
Veronika Butterweck
5  Institute for Pharma Technology, School of Life Sciences, University of Applied Sciences Northwestern Switzerland-FHNW, Muttenz, Switzerland
,
Mary Ann Foglio
1  Faculty of Pharmaceutical Sciences (FCF), State University of Campinas (Unicamp), Campinas, SP, Brazil
3  Chemical, Biological and Agricultural Research Center (CPQBA), State University of Campinas (Unicamp), Paulínia, SP, Brazil
› Author Affiliations
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2015/08600-2
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo 2016/23816-4

Abstract

Pterodon pubescens fruits are popularly used because of their analgesic and anti-inflammatory actions, which are attributed to the isolated compounds with a vouacapan skeleton. This work aimed to evaluate the antiproliferative and anti-inflammatory effects of a P. pubescens fruit dichloromethane extract and the vouacapan diterpene furan isomerʼs mixture (1 : 1) (6α-hydroxy-7β-acetoxy-vouacapan-17β-oate methyl ester and 6α-acetoxy-7β-hydroxy-vouacapan-17β-oate methyl ester isomers) in HaCaT cells using the cell migration and the BrDU incorporation assay. Levels of IL-8 were measured by ELISA after TNF-α stimulation. HPLC/DAD analysis of the extract revealed the expressive presence of vouacapan diterpene furan isomerʼs mixture. P. pubescens extract (1.5625 – 25 µg/mL) and vouacapan diterpene furan isomerʼs mixture (3.125 – 50 µM) inhibited cell proliferation as indicated by a decreased BrdU-incorporation. For the evaluation of cell migration, time-lapse microscopy was used. P. pubescens presented inhibition on cell migration at all concentrations tested (3.125 – 12.5 µg/mL), whereas for the VDFI mixture, the inhibition was only observed at the highest concentrations (12.5 and 25 µM) tested. Furthermore P. pubescens extract and vouacapan diterpene furan isomerʼs mixture significantly decreased IL-8 levels. Our results showed antiproliferative and anti-inflammatory effects on HaCaT cells treated with the extract and the vouacapan isomerʼs mixture, without affecting cell viability. These activities could be attributed to the voucapan molecular structures. In conclusion, topical products developed of P. pubescens extract or the voucapan isomerʼs mixture should be further studied as a potential product for local treatment against hyperproliferative lesions as in psoriasis vulgaris, representing an alternative treatment approach.



Publication History

Received: 01 August 2020

Accepted after revision: 28 September 2020

Publication Date:
03 November 2020 (online)

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