Abstract
UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This
technology has recently been applied to the analysis of polyphenols in food and herb
extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties
due to the presence of polyphenols. We have developed a sensitive and reproducible
UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification
and quantitation of specific polyphenolic compounds present in sage and rosemary leaves.
The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic
acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were
found in the highest concentrations among all polyphenols. In contrast, rosmarinic
acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were observed in the highest concentrations in rosemary.
Sagerinic acid, rosmanol, rosmadial, carnosol, and carnosic acid were found in abundance
in both sage and rosemary. The pentacyclic triterpenoid, corosolic acid ([M - H]¯
m/z 471.35), was detected for the first time in both plants. Of the 47 polyphenolic compounds
identified in each plant, 38 compounds were found in common in rosemary and sage.
A flavonoid compound, baicalin ([M -H]¯ m/z 445.08), was identified for the first time in S. officinalis. Also, pectolinarigenin ([M - H]¯ m/z 313.07), a dimethoxyflavone, was detected for the first time in both sage and rosemary
leaves.
Key words
Salvia officinalis
-
Rosmarinus officinalis
- Lamiaceae - rosmarinic acid - ultrasound extraction - polyphenols
