Planta Med
DOI: 10.1055/a-1240-6186
Original Papers

Profiling and Quantification of the Key Phytochemicals from the Drumstick Tree (Moringa oleifera) and Dietary Supplements by UHPLC-PDA-MS

1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
2  Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
3  Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
,
Yan-Hong Wang
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
2  Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
4  Department of Pharmaceutical Sciences, Abbottabad University of Science & Technology, Havelian, KPK, Pakistan
,
Mohammed F. Hawwal
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
2  Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
3  Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
,
Gadah A. Al-Hamoud
3  Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
,
Zulfiqar Ali
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Amar G. Chittiboyina
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
,
Ikhlas A. Khan
1  National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, University, MS, USA
2  Division of Pharmacognosy, Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, USA
› Author Affiliations
Supported by: U.S. Food and Drug Administration grant number 5U01FD004246-07
Supported by: King Saud University

Abstract

Moringa oleifera is known as a drumstick tree and is cultivated in the subtropics and tropics. It exhibits antihypertensive and antidiabetic effects. An ultra-high-performance liquid chromatography method was developed for the determination of 9 phytochemicals in M. oleifera leaves and marketed products. The efficient separation was achieved within 7 min with a temperature of 45 °C by using a C-18 column as the stationary phase and water/acetonitrile with 0.05% formic acid as the mobile phase. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The limits of detections of phenolic compounds 1 – 9 were as low as 0.2 µg/mL. The photodiode array detector at 220 and 255 nm wavelengths was recruited for quantification. The key phytochemicals were detected in the range of 0.42 to 2.57 mg/100 mg sample weight in 13 dietary supplements. This study considers the quantitative analysis for lignans in M. oleifera for the first time. Isoquercitrin (5) and quercetin 3-O-(6-O-malonyl)-β−D-glucopyranoside (6) predominates the leaves of M. oleifera with inherent degradable nature detected for compound 6. Niazirin (2) was detected in amounts between 0.010 – 0.049 mg/100 mg while compound 1 was undetectable and potentially an artifact because of the fractionation process. The characterization and confirmation of components were achieved by liquid chromatography-electrospray ionization-mass spectrometry with extractive ion monitoring for the positive and negative ion modes. The developed and validated method is robust and rapid in the conclusive quantification of phytochemicals and authentication of the Moringa samples for quality assurance.

Supporting Information



Publication History

Received: 24 February 2020

Accepted after revision: 13 August 2020

Publication Date:
21 October 2020 (online)

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