Planta Med
DOI: 10.1055/a-1214-6718
Original Papers

Codonopsis lanceolata Contributes to Ca2+ Homeostasis by Mediating SOCE and PLC/IP3 Pathways in Vascular Endothelial and Smooth Muscle Cells

Min Kyung Kim
1  Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
A Young Han
1  Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
You Kyoung Shin
1  Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
Kwang-Won Lee
2  Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea
,
1  Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
› Author Affiliations
Supported by: Basic Science Research Program through the National Research Foundation of Korea NRF-2018R1D1A1B07050048
Supported by: National Research Foundation of Korea Forest Service KFS-2016005C10-1719-AB01

Abstract

Codonopsis lanceolata has been widely used as an anti-inflammatory and anti-lipogenic agent in traditional medicine. Recently, C. lanceolata was reported to prevent hypertension by improving vascular function. This study evaluated the effects of C. lanceolata and its major component lancemaside A on cytosolic calcium concentration in vascular endothelial cells and vascular smooth muscle cells. Cytosolic calcium concentration was measured using fura-2 AM fluorescence. C. lanceolata or lancemaside A increased the cytosolic calcium concentration by releasing Ca2+ from the endoplasmic reticulum and sarcoplasmic reticulum and by Ca2+ entry into endothelial cells and vascular smooth muscle cells from extracellular sources. The C. lanceolata- and lancemaside A-induced cytosolic calcium concentration increases were significantly inhibited by lanthanum, an inhibitor of non-selective cation channels, in both endothelial cells and vascular smooth muscle cells. Moreover, C. lanceolata and lancemaside A significantly inhibited store-operated Ca2+ entry under pathological extracellular Ca2+ levels. In Ca2+-free extracellular fluid, increases in the cytosolic calcium concentration induced by C. lanceolata or lancemaside A were significantly inhibited by U73122, an inhibitor of phospholipase C, and 2-APB, an inositol 1,4,5-trisphosphate receptor antagonist. In addition, dantrolene treatment, which inhibits Ca2+ release through ryanodine receptor channels, also inhibited C. lanceolata- or lancemaside A-induced increases in the cytosolic calcium concentration through the phospholipase C/inositol 1,4,5-trisphosphate pathway. These results suggest that C. lanceolata and lancemaside A increase the cytosolic calcium concentration through the non-selective cation channels and phospholipase C/inositol 1,4,5-trisphosphate pathways under physiological conditions and inhibit store-operated Ca2+ entry under pathological conditions in endothelial cells and vascular smooth muscle cells. C. lanceolata or lancemaside A can protect endothelial cells and vascular smooth muscle cells by maintaining cytosolic calcium concentration homeostasis, suggesting possible applications for these materials in diets for preventing vascular damage.



Publication History

Received: 06 March 2020

Accepted after revision: 01 July 2020

Publication Date:
30 July 2020 (online)

Georg Thieme Verlag KG
Stuttgart · New York