Planta Med 2020; 86(12): 876-883
DOI: 10.1055/a-1203-0452
Biological and Pharmacological Activity
Original Papers

Syzygium aromaticum Reduces Diabetes-induced Glucotoxicity via the NRF2/Glo1 Pathway

Moon Ho Do
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
,
Jiwon Choi
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
,
Yoonsook Kim
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
,
Sang Keun Ha
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
2  Division of Food Biotechnology, University of Science and Technology, Daejeon, Republic of Korea
,
Guijae Yoo
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
,
Jinyoung Hur
1  Korea Food Research Institute, 245, Nongsaengmyeong-ro, Iseo-myeon, Wanju-Gun, Jeollabuk-do, Republic of Korea
2  Division of Food Biotechnology, University of Science and Technology, Daejeon, Republic of Korea
› Author Affiliations
Supported by: Korea Food Research Institute E0164400-04

Abstract

Advanced glycation end products and methylglyoxal are known to show increased levels in diabetic conditions and induce diverse metabolic disorders. However, the antiglycation ability of the bark of Syzygium aromaticum is not yet studied. In this study, we determined the inhibitory effects of S. aromaticum on AGE formation. Moreover, S. aromaticum showed breakage and inhibitory ability against the formation of AGE-collagen crosslinks. In SV40 MES13 cells, treatment with the S. aromaticum extract significantly ameliorated MG-induced oxidative stress as well as cytotoxicity. Furthermore, in the S. aromaticum extract-treated group, there was a reduction in levels of several diabetic markers, such as blood glucose, kidney weight, and urinary albumin to creatinine ratio in streptozotocin-induced diabetic rats. Treatment with the S. aromaticum extract significantly increased the expression of nuclear factor erythroid 2-related factor 2, a transcription factor involved in the expression of antioxidant enzymes. Moreover, the treatment significantly upregulated the expression of glyoxalase 1 and downregulated the expression of receptor for AGEs. These results suggest that the S. aromaticum extract might ameliorate diabetes-induced renal damage by inhibiting the AGE-induced glucotoxicity and oxidative stress through the Nrf2/Glo1 pathway.



Publication History

Received: 17 February 2020

Accepted after revision: 12 June 2020

Publication Date:
09 July 2020 (online)

Georg Thieme Verlag KG
Stuttgart · New York