Planta Med
DOI: 10.1055/a-1200-2046
Original Papers

Liquid Fungal Cocultivation as a Strategy to Access Bioactive Metabolites

Airton Damasceno Silva
1  Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil
,
Alessandra Regina Pepe Ambrozin
2  Institute of Science and Technology, Federal University of Alfenas (Unifal-MG), Poços de Caldas, MG, Brazil
,
Ana Flávia S. de Camargo
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
Felipe De Paula Nogueira Cruz
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
Leonardo Luiz Gomes Ferreira
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
Renata Krogh
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
Taynara Lopes Silva
4  Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil
,
Ilana Lopes Baratella da Cunha Camargo
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
Adriano Defini Andricopulo
3  São Carlos Institute of Physics, University of São Paulo (USP), São Carlos, SP, Brazil
,
1  Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP, Brazil
4  Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo (USP), Ribeirão Preto, SP, Brazil
› Author Affiliations
Supported by: Conselho Nacional de Desenvolvimento Científico e Tecnológico
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo #13/07600-3
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo #14/50249-8
Supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo #2019/04788-8
Supported by: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Finance Dode 001

Abstract

Fungi are a rich source of bioactive compounds. Fungal cocultivation is a method of potentiating chemical interactions and, consequently, increasing bioactive molecule production. In this study, we evaluated the bactericidal, antiprotozoal, and cathepsin V inhibition activities of extracts from axenic cultures of 6 fungi (Fusarium guttiforme, Pestalotiopsis diospyri, Phoma caricae-papayae, Colletotrichum horii, Phytophthora palmivora, and C. gloeosporioides) that infest tropical fruits and 57 extracts obtained by their cocultivation. Our results reveal that fungal cocultivation enhances the biological activity of the samples, since all extracts that were active on Gram-positive bacteria, Gram-negative bacteria, Trypanosoma cruzi, and Leishmania infantum were obtained from cocultivation. Bacterial growth is either totally or partially inhibited by 46% of the extracts. Two extracts containing mainly fusaric and 9,10-dehydrofusaric acids were particularly active. The presence of the fungus F. guttiforme in co-cultures that give rise to extracts with the highest activities against L. infantum. An axenic culture gave rise to the most active extract for the inhibition of cathepsin V; however, other coculture extracts also exhibited activity toward this biological target. Therefore, the results of the biological activities indicate that fungal cocultivation increased the biological potential of samples, likely due to the hostile and competitive environment that pushes microorganisms to produce substances important for defense and allows access to metabolic routes then silenced in milder cultivation conditions.



Publication History

Received: 27 March 2020

Accepted after revision: 05 June 2020

Publication Date:
09 July 2020 (online)

Georg Thieme Verlag KG
Stuttgart · New York