Planta Med 2020; 86(16): 1191-1203
DOI: 10.1055/a-1197-7019
Natural Product Chemistry and Analytical Studies
Original Papers

Chemical Constituents from Physalis Calyx seu Fructus and Their Inhibitory Effects against Oxidative Stress and Inflammatory Response

Hui-Xin Hu
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Lin-Tao Xu
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Hui Gao
2  Shandong Institute for Food and Drug Control, Jinan, Peopleʼs Republic of China
,
Hui Lv
3  School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, Peopleʼs Republic of China
,
Min Huang
3  School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan, Peopleʼs Republic of China
,
Kai-Li Fang
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Shu-Qi Wang
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Bao-Bing Zhao
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Xiao-Ning Wang
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Hong-Xiang Lou
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
,
Tao Shen
1  Key Lab of Chemical Biology (MOE), School of Pharmaceutical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Peopleʼs Republic of China
› Author Affiliations
Supported by: Qilu Young Scholars Program of Shandong University
Supported by: National Natural Science Foundation of China 81673558
Supported by: National Natural Science Foundation of China 81874341
Supported by: Key Research and Development Program of Shandong Province 2017GSF218049
Supported by: Multidisciplinary Research and Innovation Program of Shandong University 2020QNQT007

Abstract

Physalis Calyx seu Fructus, a traditional Chinese medicine consisting of the calyxes and fruits of Physalis alkekengi var. franchetii, has been used as therapy for inflammation-related respiratory diseases such as excessive phlegm, cough, sore throat, and pharyngitis for a long history in China. The aim of the present study was to investigate the chemical constituents of Physalis Calyx seu Fructus and identify the bioactive constituents responsible for its traditional application as therapy for inflammation-related diseases. In the present study, one new phenylpropanoid (1), two new steroids (17 and 18), together with 55 known constituents have been purified from the EtOH extract of Physalis Calyx seu Fructus. Among them, seven and twelve known constituents were isolated for the first time from Physalis Calyx seu Fructus and the genus Physalis, respectively. Fourteen constituents, including steroids [physalins (5 – 9, 12 – 14, and 15) and ergostane (21)], a sesquiterpenoid (35), alkaloids (36 and 37), and a flavonoid (44), showed inhibitory effects against oxidative stress. Ten constituents, including steroids (5, 6, 8, 13, and 15), sesquiterpenoids (34 and 35), alkaloids (37 and 41), and a flavonoid (43), were found be potential anti-inflammatory constituents of this medicinal plant. The inhibition of oxidative stress and inflammatory response may be related to the regulation of Nrf2 and nuclear factor-κB pathways. The ethnomedical use of Physalis Calyx seu Fructus as a treatment for respiratory diseases might be attributed to the combined inhibitory effects of steroids, alkaloids, sesquiterpenoids, and flavonoids against oxidative stress and inflammatory response.

Supporting Information



Publication History

Received: 04 November 2019

Accepted after revision: 03 June 2020

Publication Date:
15 July 2020 (online)

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