Planta Med 2020; 86(16): 1185-1190
DOI: 10.1055/a-1197-2898
Natural Product Chemistry and Analytical Studies
Original Papers

Phytochemical and Biological Investigation of Helianthemum nummularium, a High-Altitude Growing Alpine Plant Overrepresented in Ungulates Diets

Mathieu Agostini
1  Univ. Grenoble Alpes, CNRS, DPM, Grenoble, France
,
Isabelle Hininger-Favier
2  Univ. Grenoble Alpes, Inserm, LBFA, Grenoble, France
4  University of Colorado Anschutz Medical Campus, Aurora, CO, USA
,
Laurence Marcourt
3  School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, Geneva, Switzerland
,
Benjamin Boucherle
1  Univ. Grenoble Alpes, CNRS, DPM, Grenoble, France
,
Bifeng Gao
4  University of Colorado Anschutz Medical Campus, Aurora, CO, USA
5  Pathways Bioscience, Aurora, CO, USA
,
Brooks M. Hybertson
5  Pathways Bioscience, Aurora, CO, USA
,
Swapan K. Bose
5  Pathways Bioscience, Aurora, CO, USA
,
Joe M. McCord
5  Pathways Bioscience, Aurora, CO, USA
,
Annie Millery
6  Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, Grenoble, France
,
Maxime Rome
7  Univ. Grenoble Alpes, CNRS, SAJF, Grenoble, France
,
Emerson Ferreira Queiroz
3  School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, Geneva, Switzerland
,
3  School of Pharmaceutical Sciences, EPGL, University of Geneva, University of Lausanne, CMU, Geneva, Switzerland
,
Christiane Gallet
6  Univ. Grenoble Alpes, Univ. Savoie Mont Blanc, CNRS, LECA, Grenoble, France
,
Ahcène Boumendjel
1  Univ. Grenoble Alpes, CNRS, DPM, Grenoble, France
› Author Affiliations

Abstract

Helianthemum nummularium is a European shrub growing at high altitude where it copes with a high level of stress. It was found to be overexpressed in ungulates diets compared to more abundant surrounding plants. These elements combined with the fact that H. nummularium from the Alps has never been investigated prompted us to study the phytochemical composition of its aerial parts. The analysis of the polar extract allowed for the isolation of eight compounds: p-hydroxybenzoic acid, tiliroside, kaempferol, astragalin, quercetin, plantainoside B, quercetin-3-O-glucoside, and quercetin-3-O-glucuronide. We investigated the effect of the polar extract and isolated compounds on nuclear factor erythroid 2-related factor 2 transcription factor, which regulates the expression of a wide variety of cytoprotective genes. We found that the ethanolic extract activates the expression of nuclear factor erythroid 2-related factor 2 in a dose-dependent manner, whereas the pure compounds were much less active. The activation of the nuclear factor erythroid 2-related factor 2 pathway by the plant extract could pave the way for studies to promote healthy aging through protection of cells against oxidative stress. Moreover, the isolated compounds could be investigated alone or in combination in the perspective of making the link between the ungulateʼs preference for this plant and possible use of it for self-medication.

Supporting Information



Publication History

Received: 02 April 2020

Accepted after revision: 09 June 2020

Publication Date:
09 July 2020 (online)

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