Liraglutide Ameliorates Lipotoxicity-Induced Oxidative Stress by
                    Activating the NRF2 Pathway in HepG2 Cells

Chong-gui Zhu; Ying Luo; Hao Wang; Jun-Yi Li; Jie Yang; Ya-xin Liu; Hui-qi Qu; Bao-li Wang; Mei Zhu

doi:10.1055/a-1157-0166

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2020; 52(07): 532-539
DOI: 10.1055/a-1157-0166

Endocrine Research

Liraglutide Ameliorates Lipotoxicity-Induced Oxidative Stress by Activating the NRF2 Pathway in HepG2 Cells

Chong-gui Zhu‡

¹Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China

,

Ying Luo‡

²Tianjin Key Laboratory of Artificial Cell, Tianjin Institute of Hepatobiliary Disease, The Third Affiliated Hospital of Nankai University, Tianjin, China

,

Hao Wang‡

¹Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China

,

Jun-Yi Li

³Department of Cell Biology, Tianjin Medical University, Tianjin, China

,

Jie Yang

³Department of Cell Biology, Tianjin Medical University, Tianjin, China

,

Ya-xin Liu

¹Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China

,

Hui-qi Qu

⁴Center for Applied Genomics, The Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA

,

Bao-li Wang

⁵Key Lab of Hormones and Development (Ministry of Health), Metabolic Diseases Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin, China

,

Mei Zhu

¹Department of Endocrinology and Metabolism, Tianjin Medical University General Hospital, Tianjin, China

› Institutsangaben

Abstract

Although glucagon-like peptide-1 (GLP-1) analogue has been reported to suppress oxidative stress in non-alcoholic fatty liver disease (NAFLD), an effective therapeutic agent for NAFLD is currently unavailable. Therefore, in this study, we aimed to investigate the protective effects of the GLP-1 analogue liraglutide against lipotoxicity-induced oxidative stress in HepG2 cells and to elucidate the underlying mechanisms. HepG2 cells were cultured for 48 hours and treated with a free fatty acid (FFA) mixture: FFA mixture and liraglutide or FFA mixture, liraglutide, and exendin (9–39). Lipid accumulation was examined by oil red O staining. Oxidative stress was assessed by measuring the levels of intracellular reactive oxygen species using 2′,7′-dichlorofluorescein diacetate and thiobarbituric acid-reactive substances, whereas antioxidant capacity was assessed by measuring the activity of superoxide dismutase and catalase. Expression of the nuclear factor erythroid-2-related factor 2 (NRF2) gene and the genes encoding antioxidant enzymes was analyzed using quantitative RT-PCR. Cellular and nuclear NRF2 expression levels were assessed using immunofluorescence cell staining and western blotting. Liraglutide treatment reduced high fat-induced lipid formation and the levels of oxidative stress markers and increased antioxidant enzyme activity in HepG2 cells. Liraglutide treatment increased the mRNA expression of NRF2 target genes, induced NRF2 nuclear translocation, and increased nuclear NRF2 levels without altering NRF2 mRNA expression. Collectively, these results indicate that liraglutide exhibits a protective effect against lipotoxicity-induced oxidative stress, possibly via modulation of NRF2 and expression of antioxidant enzymes in liver cells.

Key words

GLP-1 analogues - antioxidant enzymes - NRF2 - NAFLD

Volltext

Referenzen

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