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CC BY-NC-ND 4.0 · Endosc Int Open 2019; 07(12): E1605-E1615
DOI: 10.1055/a-0990-9683
Original article
Owner and Copyright © Georg Thieme Verlag KG 2019

Gastric mucosal devitalization improves blood pressure, renin and cardiovascular lipid deposition in a rat model of obesity

Andreas Oberbach‡   *
1   Department of Medicine and Division of Gastroenterology and Hepatology. The Johns Hopkins Medical Institutions, Baltimore, Maryland, United States
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
3   Department of Cardiac Surgery, Ludwig-Maximilians-University, Munich, Germany
,
Nadine Schlichting‡   *
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
4   Department of Visceral, Transplantation, Thoracic and Vascular Surgery, Leipzig University Hospital, Leipzig, Germany
,
Yvonne Kullnick
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
,
Marco Heinrich
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
,
Stefanie Lehmann
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
5   Department of Internal Medicine, University of Leipzig, Leipzig, Germany
,
Ulf Retschlag
4   Department of Visceral, Transplantation, Thoracic and Vascular Surgery, Leipzig University Hospital, Leipzig, Germany
6   Integrated Research and Treatment Centre Obesity Diseases, Leipzig University, Leipzig, Germany
,
Maik Friedrich
2   Fraunhofer Institute for Cell Therapy and Immunology, Department of Diagnostics, Leipzig, Germany
,
Lea Fayad
1   Department of Medicine and Division of Gastroenterology and Hepatology. The Johns Hopkins Medical Institutions, Baltimore, Maryland, United States
,
Arne Dietrich
6   Integrated Research and Treatment Centre Obesity Diseases, Leipzig University, Leipzig, Germany
,
Mouen A. Khashab
1   Department of Medicine and Division of Gastroenterology and Hepatology. The Johns Hopkins Medical Institutions, Baltimore, Maryland, United States
,
Anthony N. Kalloo
1   Department of Medicine and Division of Gastroenterology and Hepatology. The Johns Hopkins Medical Institutions, Baltimore, Maryland, United States
,
Vivek Kumbhari
1   Department of Medicine and Division of Gastroenterology and Hepatology. The Johns Hopkins Medical Institutions, Baltimore, Maryland, United States
› Author Affiliations
Further Information

Publication History

submitted 08 May 2019

accepted after revision 25 June 2019

Publication Date:
25 November 2019 (online)

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Abstract

Background and study aims In lieu of the drawbacks of metabolic surgery, a method of mimicking resection of the gastric mucosa could be of value to those with obesity-related cardiovascular disease (CVD). Our study aims to investigate the effect of gastric mucosal devitalization (GMD) on blood pressure (BP) and cardiovascular lipid deposition in a rat model of obesity.

Methods GMD of 70 % of the stomach was achieved by argon plasma coagulation. GMD was compared to sleeve gastrectomy (SG) and sham (SH) in a high-fat-diet-induced rat model of obesity (48 rats). At 8 weeks, we measured noninvasive BP, renin, vessel relaxation and ghrelin receptor regulation in the aorta. In addition, we quantified cardiac lipid deposition and lipid droplet deposition in cardiac muscle and aorta.

Results GMD and SG were observed to have similar reductions in body weight, visceral adiposity, and serum lipid profile compared to SH rats. GMD resulted in a significant reduction in arterial BP compared to SH. Furthermore, there were significant reductions in plasma renin activity and percentage of phenylnephrine constriction to acetylcholine at the aortic ring in GMD rats compared to SH, providing insights into the mechanisms behind the reduced BP. Interestingly, the reduced BP occurred despite a reduction in endothelial ghrelin recteptor activation. Cardiac lipid content was significantly reduced in GMD rats. Lipid deposition, as illustrated by Nile Red stain, was reduced in cardiac muscle and the aorta.

Conclusion GMD resulted in a significant improvement in BP, renin and cardiovascular lipid deposition. GMD deserves further attention as a method of treating obesity-related CVD.

* These authors contributed equally


Supplementary material, Supplementary Table 1

 

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