Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma

Lee-Chuen Cheng; Vikneswaran Murugaiyah; Kit-Lam Chan

doi:10.1055/a-0955-9505

Planta Medica International Open, Table of Contents

CC BY-NC-ND 4.0 · Planta Medica International Open 2019; 6(01): e28-e35
DOI: 10.1055/a-0955-9505

Original Papers

Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma

Lee-Chuen Cheng

¹School of Pharmacy, University of Reading Malaysia, Persiaran Graduan, Kota Ilmu, Educity, Iskandar Puteri, Johor, Malaysia

,

Vikneswaran Murugaiyah

²Discipline of Pharmacology, School of Pharmaceutical Sciences, University of Science Malaysia (Universiti Sains Malaysia), Minden, Penang, Malaysia

,

Kit-Lam Chan

³Discipline of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, University of Science Malaysia (Universiti Sains Malaysia), Minden, Penang, Malaysia

› Author Affiliations

Abstract

An HPLC method for simultaneous determination of arenarioside (1 ), verbascoside (2), 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5) from Lippia nodiflora in rat plasma was developed and validated. The optimal chromatographic separation was achieved with a gradient mobile phase comprising 0.1% aqueous acetic acid and acetonitrile. The limit of detection was 78.1 ng/mL for 3 and 39.1 ng/mL for the other compounds (signal-to-noise ratio=3), whereas the limit of quantification was 312.5 ng/mL for 3 and 156.3 ng/mL for the other compounds (signal-to-noise ratio=12). The recovery values of compounds 1–5 ranged from 89.37–100.92%. Their accuracy values were between 96.48 and 105.81%, while their corresponding precision values were in the range of 0.75–9.06% for both intraday and inter-day analysis. The method was then applied in the first pharmacokinetic study of 1–5. Following intravenous administration, 1–5 were eliminated slowly from the body with a mean clearance value of 0.11, 0.13, 0.30, 0.09, and 0.23 L/kg h, respectively. Meanwhile, their peak plasma concentration upon oral administration was 8.97, 1.07, 1.06, 0.65, and 0.38 µg/mL, respectively. Compound 3 (5.97%) exhibited the highest absolute oral bioavailability value, followed by 1 (5.22%), 4 (3.13%), 2 (2.10%), and 5 (0.93%).

Key words

Lippia nodiflora - Verbenaceae - phenylethanoid glycosides - flavonoids - HPLC - pharmacokinetic

Full Text

References

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