Planta Med 2019; 85(02): 126-138
DOI: 10.1055/a-0755-7801
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Influence of Cranberry Extract on Tamm-Horsfall Protein in Human Urine and its Antiadhesive Activity Against Uropathogenic Escherichia coli

Birte Scharf
1  University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Münster, Germany
,
Jandirk Sendker
1  University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Münster, Germany
,
Ulrich Dobrindt
2  University Hospital Münster, Institute of Hygiene, Münster, Germany
,
Andreas Hensel
1  University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Münster, Germany
› Author Affiliations
Further Information

Publication History

received 17 August 2018
revised 26 September 2018

accepted 04 October 2018

Publication Date:
12 October 2018 (eFirst)

Abstract

LC-MS characterized cranberry extract from the fruits of Vaccinium macrocarpon inhibited under in vitro conditions the bacterial adhesion of Escherichia coli strain 2980 uropathogenic E. coli (UPEC strains UTI89, NU14) to T24 bladder cells and adhesion of UPEC strain CFT073 to A498 kidney cells in a concentration-dependent manner. Within a biomedical study, urine samples from 16 volunteers (8 male, 8 female) consuming cranberry extract for 7 d (900 mg/d) were analyzed for potential antiadhesive activity against UPEC by ex vivo experiments. Results indicated inhibition of adhesion of UPEC strain UTI89 to human T24 bladder cells. Subgroup analysis proved significant inhibition of bacterial adhesion in case of urine samples obtained from male volunteers while female urine did not influence the bacterial attachment. Differences between antiadhesive capacity of urine samples from male/female volunteers were significant. Protein analysis of the urine samples indicated increased amounts of Tamm-Horsfall protein (THP, syn. uromodulin) in the active samples. Inhibition of bacterial adhesion by the urine samples was correlated to the respective amount of THP. As it is known that THP, a highly mannosylated glycoprotein, strongly interacts with FimH of UPEC, this will lead to a decreased interaction with uroplakin, a FimH-binding transmembrane protein of urothelial lining cells. From these data it can be concluded that the antiadhesive effect of cranberry after oral intake is not only related to the direct inhibition of bacterial adhesins by extract compounds but is additionally due to an induction of antiadhesive THP in the kidney.