Planta Med 2018; 84(12/13): 947-952
DOI: 10.1055/a-0632-2204
Natural Product Chemistry and Analytical Studies
Rapid Communications
Georg Thieme Verlag KG Stuttgart · New York

A Cyclotide Isolated from Noisettia orchidiflora (Violaceae)[*]

Antonio Fernández Bobey**
1   NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil
,
Meri Emili Ferreira Pinto**
1   NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil
,
Eduardo Maffud Cilli
1   NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil
,
Norberto Peporine Lopes
2   NPPNS, Faculty of Pharmaceutical Sciences of Ribeirão Preto-USP, The University of São Paulo-USP, Ribeirão Preto-São Paulo, Brazil
,
Vanderlan da Silva Bolzani
1   NuBBE, Institute of Chemistry, São Paulo State University-UNESP, Araraquara-São Paulo, Brazil
› Author Affiliations
Further Information

Publication History

received 30 January 2018
revised 14 May 2018

accepted 16 May 2018

Publication Date:
29 May 2018 (online)

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Abstract

Biologically active cyclotides have been found on some flowering plants species and are involved in the role of the plant protection. As part of studies focusing on peptides from Brazilian plant species, we are reporting the detection by LC-MS of several cyclotides from leaves and stems of Noisettia orchidiflora (Violaceae). From stems it was possible to isolate and characterize a cyclotide named Nor A. Its primary structure (amino acid sequence) was established by MALDI-TOF-MS, based on the y- and b-type ion series, after reduction and alkylation reactions, as well as enzymatic digestion using the enzymes endoproteinase glutamic acid (endoGlu-C), trypsin, and chymotrypsin. Furthermore, the amino acid analysis was also described.

* Dedicated to Professor Dr. Robert Verpoorte in recognition of his outstanding contribution to natural products research.


** These authors contributed equally to this work.


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