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Planta Med 2018; 84(14): 1038-1044
DOI: 10.1055/a-0578-8689
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay for Determination of Homoharringtonine

Benyakan Pongkitwitoon   *
1   Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
,
Seiichi Sakamoto   *
2   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
,
Rika Nagamitsu
2   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
,
Waraporn Putalun
3   Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, Thailand
4   Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research University-Khon Kaen University, Khon Kaen, Thailand
,
Hiroyuki Tanaka
2   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
,
Satoshi Morimoto
2   Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
› Author Affiliations
Further Information

Publication History

received 22 November 2017
revised 25 January 2018

accepted 15 February 2018

Publication Date:
28 February 2018 (online)

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Abstract

Homoharringtonine (HHT), also known as omacetaxine, is a natural compound found in the genus Cephalotaxus and is a promising pharmaceutical drug used for the treatment of chronic or accelerated phase chronic myeloid leukemia. As a tool for the quantitative determination of HHT, a specific monoclonal antibody against HHT (MAb 6A1) was generated by conjugates prepared via sodium periodate-mediated oxidation. The developed indirect competitive enzyme-linked immunosorbent assay (icELISA) using MAb 6A1 was found to be highly specific and sensitive with a limit of detection for HHT of 48.8 ng/mL. Validation assays to evaluate precision and accuracy of the method were conducted by the use of intra- and inter-assay analysis, recovery test, and comparison analysis between the amounts of HHT determined by ELISA and high-performance liquid chromatography. These results revealed that the established icELISA using MAb 6A1 is specific, sensitive, and reliable enough to be applied to the qualitative analysis for HHT. Furthermore, the results of this study support the usefulness of sodium periodate as a reagent for the conjugation between Cephalotaxus alkaloids and proteins for producing specific antibodies.

Key words

Cephalotaxus harringtonii - enzyme-linked immunosorbent assay - homoharringtonine - monoclonal antibody - sodium periodate-mediated conjugation - Taxaceae

* These authors contributed equally to this work.


Supporting Information

    Dose-response curves of icELISA using MAb 6A1 to determine IC50 for CR of HT and cephalotaxine are available as Supporting Information.

  • Supporting Information
 

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