CC BY-NC-ND 4.0 · Journal of Coloproctology 2016; 36(04): 189-195
DOI: 10.1016/j.jcol.2016.04.007
Original Article

Effects of hydroalcoholic extract of Ziziphus jujuba on acetic acid induced ulcerative colitis in male rat (Rattus norvegicus)

Efeitos do extrato hidroalcoólico de Ziziphus jujuba na colite ulcerativa induzida pelo ácido acético em rato macho (Rattus norvegicus)
Nader Tanideh
a  Shiraz University of Medical Sciences, Stem Cell and Transgenic Technology Research Center, Shiraz, Iran
b  Shiraz University of Medical Sciences, School of Medicine, Department of Pharmacology, Shiraz, Iran
,
Akram Jamshidzadeh
c  Shiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, Iran
,
Ali Ghanbari Saghesloo
c  Shiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, Iran
,
Farhad Rahmanifar
d  Shiraz University, School of Veterinary Medicine, Department of Anatomy, Shiraz, Iran
,
Maral Mokhtari
e  Shiraz University of Medical Sciences, School of Medicine, Department of Pathology, Shiraz, Iran
,
Omid Koohi-Hosseinabadi
f  Shiraz University of Medical Sciences, Center of Comparative and Experimental Medicine, Shiraz, Iran
,
Mahmood Omidi
c  Shiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, Iran
,
Asma Najibi
c  Shiraz University of Medical Sciences, School of Pharmacy, Department of Pharmacology Toxicology, Shiraz, Iran
› Author Affiliations

Abstract

Objective To investigate the effects of hydroalcoholic extract of Ziziphus jujuba on the histopathological, tissue oxidative stress and inflammation plus to antioxidant pathways of colon tissue in rat with induced Ulcerative colitis.

Materials and methods Ulcerative colitis was induced in 80 rats those divided into 8 equal groups. Group 1 and 2 were negative controls receiving 1 mL/day of normal saline in enema and oral; group 3 and 4 as positive control 1 and 2 received 10 mg/kg of intra-colonic asacol and oral mesalazine; groups 5 and 6 received 20% and 40% of hydroalcoholic extract of Z. jujuba trans-rectally; group 7 and 8 received 1500 and 3000 mg/kg of hydroalcoholic extract of Z. jujuba orally, respectively. After 7 days, animals were evaluated for colon tissue histopathology, levels of malondialdehyde and IL-1β, and activities of superoxide dismutase, glutathione peroxidase and myeloperoxidase in colon tissue.

Results Hydroalcoholic extract of Z. jujuba in both forms of trans-rectal and oral administration especially in the higher doses could result into a more healing effect in damaged colonic tissue, more reduce glutathione peroxidase and IL-1β level. Also, these two doses (gel 40% and oral 3000 mg/kg) could more decrease the myeloperoxidase activity and stimulate superoxide dismutase and glutathione peroxidase activities. Also, gel 40% in transrectal administration was more potent than administration 3000 mg/kg in oral.

Conclusion The results of the present study indicated that Z. jujube may be considered as a treatment of choice for Ulcerative colitis especially in gel form and also in dose-dependent pattern.

Resumo

Objetivo Investigar os efeitos do extrato hidroalcoólico de Ziziphus jujuba no estresse oxidativo em tecido ao nível histopatológico e na inflamação, juntamente com as vias antioxidantes em tecido de cólon em ratos com colite ulcerativa induzida.

Materiais e métodos: Induzimos colite ulcerativa em 80 ratos, divididos em 8 grupos iguais. Os grupos 1 e 2 eram controles negativos que receberam 1 mL/dia de salina normal em enema e por via oral; os grupos 3 e 4 eram controles positivos para 1 e 2 e receberam 10 mg/kg de asacol por via intracolônica e mesalazina oral; os grupos 5 e 6 receberam gel a 20% e 40% de extrato hidroalcoólico de Z. jujuba por via trans-retal; os grupos 7 e 8 receberam 1500 e 3000 mg/kg de extrato hidroalcoólico de Z. jujuba por via oral, respectivamente. Transcorridos 7 dias, os animais foram avaliados para histopatologia de tecido de cólon, níveis de malonildialdeído e IL-1β, e atividades de superóxido dismutase, glutátion peroxidase e mieloperoxidase no tecido colônico.

Resultados O uso do extrato hidroalcoólico de Z. jujuba, tanto na forma transretal como oral, e em especial nas doses mais altas, resultou em um efeito de cicatrização mais intensa no tecido colônico lesionado, e em maior redução nos níveis de glutátion peroxidase IL-1β. Além disso, essas duas doses (gel a 40% e 3000 mg/kg por via oral) diminuíram ainda mais a atividade de mieloperoxidase e estimularam as atividades de superóxido dismutase e glutátion peroxidase. Outro achado do estudo foi que o gel a 40% por administração trans-retal se mostrou mais potente do que a administração oral de 3000 mg/kg.

Conclusão Os resultados do presente estudo sugerem que Z. jujuba pode ser considerado como tratamento de escolha para colite ulcerativa, sobretudo em forma de gel e também em um padrão proporcional à dose administrada.



Publication History

Received: 01 March 2016

Accepted: 20 April 2016

Publication Date:
17 February 2021 (online)

© 2016. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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