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CC BY-NC-ND 4.0 · Journal of Coloproctology 2013; 33(03): 118-125
DOI: 10.1016/j.jcol.2013.06.002
Original article

APC protein immunoexpression in colorectal adenoma and adenocarcinoma

Imunoexpressão da proteína APC nos tecidos de adenoma e de adenocarcinoma colorretais

Authors

  • Vivian Sati Oba Bourroul

    a   Service of Digestive System Surgery of Hospital do Servidor Público Estadual (HSPE), São Paulo, SP, Brazil
    b   Service of Digestive System Surgery of Hospital Estadual Mário Covas, Santo André, SP, Brazil

  • Guilherme Muniz Bourroul

    c   Service of Digestive System Surgery of Hospital Estadual Mário Covas, Santo André, SP, Brazil

  • Giovanna Canato Toloi

    d   Faculdade de Medicina do ABC, São Paulo, SP, Brazil

  • Rogério Tadeu Palma

    a   Service of Digestive System Surgery of Hospital do Servidor Público Estadual (HSPE), São Paulo, SP, Brazil
    e   Discipline of Service of Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, Brazil

  • Celina Tizuko Fujiyama Oshima

    f   Laboratory of Molecular Pathology of Escola Paulista de Medicina da Universidade Federal de São Paulo (EPM-UNIFESP), São Paulo, SP, Brazil

  • Thiago Simão Gomes

    g   Laboratory of Molecular Pathology, Departament of Pathology, EPM-UNIFESP, São Paulo, SP, Brazil

  • Sílvia Saiuli Miki Ihara

    g   Laboratory of Molecular Pathology, Departament of Pathology, EPM-UNIFESP, São Paulo, SP, Brazil

  • Jaques Waisberg

    h   General and Digestive System Surgery of Faculdade de Medicina do ABC, São Paulo, SP, Brazil
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Abstract

Background activation of the Wnt pathway by mutated APC gene is considered the initial event in colorectal carcinogenesis. The identification of these mutations can improve the specific treatment of the adenocarcinoma.

Objective detect and evaluate wild-type APC protein in tissue from colorectal adenoma, adenocarcinoma and adjacent mucosa.

Methods 42 patients that underwent surgery for adenocarcinoma and 53 patients with resected adenomas were studied. Tissue samples from the adenocarcinoma were obtained from the tumor and from adjacent non-neoplastic mucosa located 10 cm from the proximal margin of the tumor. Adenoma tissue was obtained from representative areas. Blocks of tissue microarray (TMA) were submitted to immunohistochemistry with anti-APC, with readings of positivity and intensity of immunostaining and the score of immune expression of APC protein was obtained.

Results the APC protein immune expression score showed a significantly lower expression of APC protein in the adenoma when compared with the adenocarcinoma (p < 0.0001) and adjacent mucosa (p < 0.0001). The APC protein immune expression score in the colorectal mucosa and adjacent to the adenocarcinoma showed no significant difference (p = 0.24).

Conclusions the finding of decreased expression of APC protein in adenoma tissue may indicate that the mutated APC gene may contribute to the changes in the adenoma-carcinoma process of carcinogenesis sequence. The strong expression of protein APC in tissues from the carcinoma and adjacent mucosa suggests that in most patients in this series, the mutation of the APC gene did not participate in the oncogenesis mechanism.

Resumo

Racional a ativação da via Wnt pelo gene APC mutado é considerado evento inicial da carcinogênese colorretal. A identificação dessas mutações pode tornar o tratamento do adenocarcinoma mais específico.

Objetivo detectar e avaliar a proteína APC não mutada em tecidos de adenoma, adenocarcinoma e mucosa adjacente.

Método estudados 42 doentes operados de adenocarcinoma e 53 com adenomas ressecados. Tecidos de adenocarcinoma foram obtidas da neoplasia e da mucosa adjacente não neoplásica situadas a 10 cm da margem proximal do tumor. Tecidos do adenoma foram obtidas de área representativa. Blocos de tissue microarray (TMA) foram submetidos a imuno-histoquímica com anticorpo anti-APC. Avaliadas a positividade e intensidade da expressão e obtidos escores da imunoexpressão da proteína APC.

Resultados o escore da imunoexpressão da proteína APC no adenoma foi significantemente menor do que no adenocarcinoma (p < 0,0001) e na mucosa adjacente (p < 0,0001). O escore da imunoexpressão da proteína APC na mucosa adjacente e no adenocarcinoma não mostraram diferença significante (p = 0,24).

Conclusões a menor expressão da proteína APC no adenoma pode indicar que o gene APC mutado participa das alterações do processo adenoma-carcinoma. A forte expressão da proteína APC no CCR e na mucosa adjacente sugerem que a mutação do gene APC não participou da oncogênese.

Keywords

Colorectal neoplasms Carcinoma - Adenoma APC Gene - Immunohistochemistry Biological tumor markers

Palavras-chave

Neoplasias colorretais Carcinoma - Adenoma Gene APC - Imunoistoquímica - Marcadores biológicos de tumor


Publication History

Received: 12 May 2013

Accepted: 10 June 2013

Article published online:
17 February 2021

© 2013. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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