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DOI: 10.1016/j.jcol.2012.10.001
Autophagy and proinflammatory cytokine expression in the intestinal mucosa and mesenteric fat tissue of patients with Crohn's disease
Autofagia e expressão de citocinas pró-inflamatórias na mucosa intestinal e no tecido mesenterial de pacientes com doença de Crohn![](https://www.thieme-connect.de/media/10.1055-s-00050098/201301/lookinside/thumbnails/10-1016-j-jcol-2012-10-001_s223793631300004x-1.jpg)
Abstract
Background Recently, mesenteric fat has been proposed to play a role in the pathophysiol- ogy of Crohn's disease (CD), as fat hypertrophy is detected close to the affected intestinal area; however, there are few studies regarding autophagy and creeping fat tissue in CD.
Objective Evaluate autophagy-related proteins and proinflammatory cytokines in intestinal mucosa and mesenteric fat in patients with CD and controls.
Patients and methods Ten patients with CD, eight with non-inflammatory disease who underwent surgery, and eight with normal ileocolonoscopy were studied. The expression of LC3-II, TNF-α and IL-23 was determined by immunoblot of protein extracts. In addition, total RNA of LC3 and Atg16-L1 were determined using RT-PCR.
Results The expression of LC3-II was significantly lower in the mesenteric tissue of CD when compared to controls (p < 0.05). In contrast, the intestinal mucosa of the CD group had higher levels of LC3-II (p < 0.05). However, mRNA expression of autophagy-related pro- teins was similar when compared to mesenteric fat groups. TNF-α and IL-23 expressions were higher in intestinal mucosa of CD than in control (p < 0.05).
Conclusion These findings suggest a defect in the autophagic activity of the creeping fat tissue in CD, which could be involved with the maintenance of the inflammatory process in the intestinal mucosa.
Resumo
Introdução Recentemente, tem se proposto que o tecido mesenterial possa participar da fisiopatologia da DC, uma vez que é notória a hipertrofia da gordura mesenterial próxima ao segmento intestinal afetado pela doença. Entretanto, há poucos estudos relacionando autofagia e tecido mesenterial na DC.
Objetivo Avaliar autofagia e citocinas na mucosa intestinal e no mesentério de pacientes com DC.
Pacientes e métodos Dez pacientes com DC, oito sem doença inflamatória intestinal que foram submetidos à cirurgia, e oito com ileocolonoscopia normal, foram estudados. As ex- pressões de LC3-II, TNF-α e IL-23 foram determinadas por imunoblot de extrato protéico total. Além disso, expressão gênica de LC3 e de Atg16-L1 foi realizada por RT-PCR.
Resultados A expressão de LC3-II foi significativamente menor no tecido mesenterial de pa- cientes com DC quando comparada à dos controles (p < 0,05); as amostras de tecido intes- tinal do grupo DC apresentaram maior expressão de LC3-II (p < 0,05). Entretanto, as expres- sões gênicas relacionadas à autofagia foram similares nos grupos de tecido mesenterial. Os níveis de TNF-α e de IL-23 foram maiores na mucosa intestinal do grupo CD (p < 0,05).
Conclusão Estes achados sugerem alteração da autofagia no mesentério na DC, o que pode estar envolvido com a manutenção da inflamação na mucosa intestinal.
Palavras-chave
Doença de Crohn - Doença inflamatória intestinal - Autofagia - Citocinas - Gordura mesenterialPublication History
Received: 01 June 2012
Accepted: 10 October 2012
Article published online:
05 February 2021
© 2013. Sociedade Brasileira de Coloproctologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)
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