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Stimulative influence of germination and growth of maize seedlings originating from aged seeds by 2,4-D potencies
Received21 November 2011
revised04 May 2013
accepted09 May 2013
20 December 2017 (online)
Background: The 2,4-D (2,4-dichlorophenoxyacetic acid) is using as a growth regulator in tissue culture media. Maize seeds have poor ability to maintain germination rate in the long term.
Objective: To examine the possible restorative effect of homeopathic 2,4-D potencies on maize seedlings originating from seeds damaged by accelerated aging.
Methods: Seeds of four maize lines were subjected to accelerated aging stress treatment. Seed samples were treated with distilled water (control) and a range of potencies of 2,4-D: 3C, 3.75C, 4.5C, 5.25C and 6C. The germination capacity, fresh substance (FS) and length of root and shoot were determined. Hydrolysis and biosynthesis, GSH/GSSG ratio and redox capacity were calculated.
Results: Induced seed aging decreased germination rate and growth of seedlings. 2,4-D potencies did not have a statistically significant effect on germination. However, there were statistically significant effects on FS production, root and shoot length and redox capacity. The 3C potency had the largest effect on the FS accumulation, 4.5C increased root and shoot length, compared to control (statistically significant). The GSH/GSSG ratio and the redox capacity were decreased by aging. The 3C and 4.5C potencies tended to reverse the GSH/GSSG ratio (statistically significant) in the root and shoot, (i.e., shifted the redox balance to the reduced state).
Conclusion: Homeopathic potencies of 2,4-D appear to have a beneficial effect on artificially aged maize seeds: they stimulate growth through better substance conversion from seed rest, and shift the redox capacity towards a reduced environment. Further work is required to determine if this is an useful means of improving maize seed germination and growth.
- 1 Bewely J.D., Black M. Seeds: physiology of development and germination. New York: Plenum Press; 1985.
- 2 Bewley J.D. Seed germination and dormancy. Plant Cell 1997; 9: 1055-1066.
- 3 Tommasi F., Paciolla C., de Pinto M.C., De Gara L. A comparative study of glutathione and ascorbate metabolism during germination of Pinus pinea L. seeds. J Exp Bot 2001; 52: 1647-1654.
- 4 Smiri M., Chaoui A., Rouhier N. et al. Cadmium induced mitochondrial redox changes in germinating pea seed. Biometals 2010; 23: 973-984.
- 5 McDonald M.B. Seed deterioration: physiology, repair and assessment. Seed Sci Technol 1999; 27: 177-237.
- 6 Leprince O., Atherton N.M., Deltour R., Hendry G.A.F. The involvement of respiration in free radical processes during loss of desiccation tolerance in germinating Zea mays L.: an electron paramagnetic resonance study. Plant Physiol 1994; 104: 1333-1339.
- 7 Torres M., De Paula M., Pérez-Otaola M., Darder M., Frutos G., Martínez-Honduvilla C.J. Ageing-induced changes in glutathione system of sunflower seeds. Physiol Plant 1997; 101: 807-814.
- 8 Schafer F.Q., Buettner G.R. Redox environment of the cell as viewed through the redox state of the glutathione disulfide/glutathione couple. Free Radic Bio Med 2001; 30: 1191-1212.
- 9 Willekens H., Chamnongpol S., Davey M. et al. Catalase is a sink for H2O2 and is indispensable for stress defense in C3 plants. EMBO J 1997; 16: 4806-4816.
- 10 Betti L., Trebbi G., Majewsky V. et al. Use of homeopathic preparations in phytopathological models and in field trials: a critical review. Homeopathy 2009; 98: 244-266.
- 11 Hamman B., Koning G., Him Lok K. Homeopathically prepared gibberellic acid and barley seed germination. Homeopathy 2003; 92: 140-144.
- 12 Cox C. 2,4-D toxicology. J Pest Ref 1999; 19: 14-19.
- 13 Raghavan V. Role of 2,4-dichlorophenoxyacetic acid (2,4-D) in somatic embryogenesis on cultured zygotic embryos of Arabidopsis: cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2,4-D. Am J Bot 2004; 91: 1743-1756.
- 14 Ghasemnezhad A., Mousavizadeh S.J., Mashayekhi K. A study on evening-primrose (Oenothera biennis L.) callus regeneration and somatic embryogenesis. Iran J Biotechnol 2011; 9: 31-36.
- 15 Bukowska B. Toxicity of 2,4-dichlorophenoxyacetic acid – molecular mechanisms. Pol J Environ Stud 2006; 15: 365-374.
- 16 Morre D.J., Brightman A.O., Wu L.Y., Barr R., Leak B., Crane F.L. Role of plasma membrane redox activities in elongation growth in plants. Physiol Plant 1988; 73: 187-193.
- 17 Ries S.K. Herbicides as growth regulators. Weeds Today 1971; 2: 6-8.
- 18 Kasai F., Bayer D.E. Effects of 2,4-dichlorophenoxyindolacetic acid, antiauxins and metabolic perturbations on cytoplasmic and vacuolar pH of corn root tips measured by in vivo 31P-NMR. Pestic Biochem Physiol 1995; 51: 161-169.
- 19 Hariharan M., Unnikrishnan K. 2,4-D treatment of seed of Trigonella foenum-graecum can enhance fruit and seed development in plants raised from them. Seed Sci Technol 1983; 11: 307-315.
- 20 Woltz J.M., TeKrony D.M. Accelerated ageing test for corn seed. Seed Technol 2001; 23: 21-34.
- 21 Shimabukuro R.H., Walsh W.C., Hoerauf R.A. Reciprocal antagonism between the herbicides, diclofop-methyl and 2,4-D, in corn and soybean tissue culture. Plant Physiol 1986; 80: 612-617.
- 22 Close K.R., Ludeman L.A. The effect of auxin-like plant growth regulators and osmotic regulation on induction of somatic embryogenesis from elite maize lines. Plant Sci 1987; 52: 81-89.
- 23 ISTA. International rules for seed testing: the germination test. Bassersdorf, Switzerland: International Seed Testing Association; 2007: 5A-31 Chap 5.
- 24 Dragicevic V., Sredojevic S., Djukanovic L., Srebric M., Pavlov M., Vrvic M. The stimulatory effects of 2,4-D as hormetic on maize seedling's growth. Maydica 2007; 52: 307-310.
- 25 de Kok L.J., de Kan P.J.L., Tanczos G., Kupier J.C. Sulphate induced accumulation of glutathione and frost-tolerance of spinach leaf tissue. Physiol Plant 1981; 53: 435-438.
- 26 Ajayi S.A., Fakorede M.A.B. Physiological maturity effects on seed quality, seedling vigour and mature plant characteristics of maize in a tropical environment. Seed Sci Technol 2000; 28: 301-319.
- 27 Ganguli S., Sen-Mandi S. Effects of ageing on amylase activity and scutellar cell structure during imbibition in wheat seed. Ann Bot 1993; 71: 411-416.
- 28 Aoki N., Scofield G.N., Wang X.-D., Offler C.E., Patrick J.W., Furbank R.T. Pathway of sugar transport in germinating wheat seeds. Plant Physiol 2006; 141: 1255-1263.
- 29 Leonova S., Grimberg Å., Marttila S., Stymne S., Carlsson A.S. Mobilization of lipid reserves during germination of oat (Avena sativa L.), a cereal rich in endosperm oil. J Exp Bot 2010; 61: 3089-3099.
- 30 Subedi C.K., Bhattarai T. Effect of gibberellic acid on reserve food mobilization of maize (Zea mays L. var Arun-2) endosperm during germination. Him J Sci 2003; 2: 99-102.
- 31 Calabrese E.J., Baldwin L.A. Chemical hormesis: its historical foundations as a biological hypothesis. Human Exp Toxicol 2000; 19: 2-31.
- 32 Dragicevic V., Sredojevic S., Spasic M.B. Introduction of the interdependence between the glutathione half-cell reduction potential and thermodynamic parameters during accelerated aging of maize seeds. J Serb Chem Soc 2010; 75: 323-331.
- 33 Benson E.E. Cryopreservation of phytodiversity: a critical appraisal of theory & practice. Crit Rev Plant Sci 2008; 27: 141-219.