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Homeopathy 2010; 99(01): 51-56
DOI: 10.1016/j.homp.2009.11.005
Original Paper
Copyright © The Faculty of Homeopathy 2009

Basophil models of homeopathy: a sceptical view

Madeleine Ennis
Subject Editor:
Further Information

Publication History

Received29 September 2009
revised05 November 2009

accepted05 November 2009

Publication Date:
16 December 2017 (online)

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This paper examines the activation and inhibition of activation of human basophils. After a brief description of human basophils, different methods to determine basophil activation are discussed with a special emphasis on the use of flow cytometric methods, as these circumvent the potential problems of assays based on the loss of colour by activated basophils. The activation of human basophils by ultra-high dilutions of anti-IgE is discussed. The majority of the paper describes the inhibition of basophil activation by ultra-high dilutions of histamine. The results from published papers are described and discussed.

After over 20 years research trying to find out if high dilutions of histamine have a negative feedback effect on the activation of basophils by anti-IgE, what do we know? The methods are poorly standardized between laboratories – although the same is true for conventional studies. Certainly there appears to be some evidence for an effect – albeit small in some cases – with the high dilutions in several different laboratories using the flow cytometric methodologies. After standardization of a number of parameters, it is recommended that a multi-centre trial be performed to hopefully put an end to this “never-ending story”.

Keywords

Homeopathy - In vitro - Basophils
 

  • References

  • 1 Davenas E., Beauvais F., Amara J. et al. Human basophil degranulation triggered by very dilute antiserum against IgE. Nature 1988; 333: 816-818.
    CrossrefPubMedGoogle Scholar
  • 2 Ovelgonne J.H., Bol A.W., Hop W.C., van Wijk R. Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties. Experientia 1992; 48: 504-508.
    CrossrefPubMedGoogle Scholar
  • 3 Hirst S.J., Hayes N.A., Burridge J., Pearce F.L., Foreman J.C. Human basophil degranulation is not triggered by very dilute antiserum against human IgE. Nature 1993; 366: 525-527.
    CrossrefPubMedGoogle Scholar
  • 4 Witt C.M., Bluth M., Albrecht H., Weisshuhn T.E., Baumgartner S., Willich S.N. The in vitro evidence for an effect of high homeopathic potencies–a systematic review of the literature. Complement Ther Med 2007; 15: 128-138.
    CrossrefPubMedGoogle Scholar
  • 5 Karl and Veronica Carstens-Foundation. The HomBRex-database. www.carstens-stiftung.de/hombrex 2008
    PubMedGoogle Scholar
  • 6 Lichtenstein L.M., Gillespie E. Inhibition of histamine release by histamine controlled by H2 receptor. Nature 1973; 244: 287-288.
    CrossrefPubMedGoogle Scholar
  • 7 Sainte-Laudy J., Sambucy J.L., Belon P. Biological activity of ultra low doses: I/ effect of ultra low doses of histamine on human basophil degranulation triggered by D. Pteronyssinus extract ultra low doses. Taylor & Francis; 1991. 127–138.
    Google Scholar
  • 8 Bellavite P., Ortolani R., Pontarollo F., Conforti A., Pitari G. Immunology and homeopathy. 5. The rationale of the ‘Simile’. Evid Based Complement Alternat Med 2007; 4: 149-163.
    CrossrefPubMedGoogle Scholar
  • 9 Gilbert H.S., Ornstein L. Basophil counting with a new staining method using alcian blue. Blood 1975; 46: 279-286.
    PubMedGoogle Scholar
  • 10 Lichtenstein L.M., Norman P.S., Connell J.T. Comparison between skin-sensitizing antibody titers and leukocyte sensitivity measurements as an index of the severity of ragweed hay fever. J Allergy 1967; 40: 160-167.
    CrossrefPubMedGoogle Scholar
  • 11 Crockard A.D., Ennis M. Laboratory-based allergy diagnosis; should we go with the flow?. Clin Exp Allergy 2001; 31: 957-977.
    PubMedGoogle Scholar
  • 12 Brown V., Ennis M. In: Pawankar Ruby, Holgate Stephen T., Rosenwasser Lanny J. CD203c for Allergy Diagnosis. Allergy Frontiers:Diagnosis and Health Economics Series: Allergy Frontiers. Vol 4. 2009: 183-199.
  • 13 Crockard A.D., Ennis M. Basophil histamine release tests in the diagnosis of allergy and asthma. Clin Exp Allergy 2001; 31: 345-350.
    CrossrefPubMedGoogle Scholar
  • 14 Bochner B.S., Sterbinsky S.A. Altered surface expression of CD11 and Leu 8 during human basophil degranulation. J Immunol 1991; 146: 2367-2373.
    PubMedGoogle Scholar
  • 15 Michova A., Abugalia M., Ivanova T., Nikolov G., Taskov H., Petrunov B. Comparision of two-flow cytometry methods for basophil degranulation in patients sensitized to grass pollen. Allergy 2006; 61: 1078-1083.
    CrossrefPubMedGoogle Scholar
  • 16 Bühring H.J., Simmons P.J., Pudney M. et al. The monoclonal antibody 97A6 defines a novel surface antigen expressed on human basophils and their multipotent and unipotent progenitors. Blood 1999; 94: 2343-2356.
    PubMedGoogle Scholar
  • 17 Hennersdorf F., Florian S., Jakob A. et al. Identification of CD13, CD107a, and CD164 as novel basophil activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res 2005; 15: 325-335.
    CrossrefPubMedGoogle Scholar
  • 18 Hauswirth A.W., Sonneck K., Florian S. et al. Interleukin-3 promotes the expression of E-NPP3/CD203C on human blood basophils in healthy subjects and in patients with birch pollen allergy. Int J Immunopathol Pharmacol 2007; 20: 267-278.
    CrossrefPubMedGoogle Scholar
  • 19 De Week A.L., Sanz M.L., Gamboa P.M. et al. Diagnostic tests based on human basophils: more potentials and perspectives than pitfalls. II. Technical issues. J Investig Allergol Clin Immunol 2008; 18: 143-155.
    PubMedGoogle Scholar
  • 20 Kleine-Tebbe J., Erdmann S., Knol E.F., MacGlashan Jr. D.W., Poulsen L.K., Gibbs B.F. Diagnostic tests based on human basophils: potentials, pitfalls and perspectives. Int Arch Allergy Immunol 2006; 141: 79-90.
    CrossrefPubMedGoogle Scholar
  • 21 Poitevin B., Davenas E., Benveniste J. In vitro immunological degranulation of human basophils is modulated by lung histamine and Apis mellifica. Br J Clin Pharmacol 1988; 25: 439-444.
    CrossrefPubMedGoogle Scholar
  • 22 Poitevin B., Aubin M.J., Benveniste J. Approach to a quantitative analysis of the effect of Apis mellifica on the in vitro human basophil degranulation. J Innov Tech. Biol Med 1986; 7: 64-68.
    PubMedGoogle Scholar
  • 23 ‘High-dilution’ experiments a delusion. Nature 1988; 334: 287-290.
    CrossrefPubMedGoogle Scholar
  • 24 Benveniste J., Davenas E., Ducot B., Cornillet B., Poitevin B., Spira A. L'agitation de solutions hautement diluées n'induit pas d'activité biologique spécifique. C.R Acad Sci Paris, Série II 1991; 312: 461-466.
    PubMedGoogle Scholar
  • 25 Knol E.F., Mul F.P., Kuijpers T.W., Verhoeven A.J., Roos D. Intracellular events in anti-IgE nonreleasing human basophils. J Allergy Clin Immunol 1992; 90: 92-103.
    CrossrefPubMedGoogle Scholar
  • 26 Sainte-Laudy J. Standardization of basophil degranulation for pharmacological studies. J Immunol Methods 1987; 98: 279-282.
    CrossrefPubMedGoogle Scholar
  • 27 Sainte-Laudy J., Belon P. Biological activity of ultra low doses: II/ Effect of ultra low doses of histamine on human basophil degranulation triggered by anti-IgE. Ultra low doses. Taylor & Francis; 1991. 139–143.
    Google Scholar
  • 28 Belon P., Cumps J., Ennis M., Mannaioni P.F., Roberfroid M., Sainte-Laudy J. et al. Histamine dilutions modulate basophil activation. Inflamm Res. 2004; 53: 181-188.
    CrossrefPubMedGoogle Scholar
  • 29 Sainte-Laudy J., Belon P. Analysis of immunosuppressive activity of serial dilutions of histamine on human basophil activation by flow cytometry. Inflamm Res 1996; 45 (Suppl. 01) S33-S34.
    CrossrefPubMedGoogle Scholar
  • 30 Sainte-Laudy J., Boudjedaini N., Belon P. Differential effect of storage on molecular and ultra-molecular dilutions of histamine. Inflamm Res 2009; 58 (Suppl. 01) 30-31.
    PubMedGoogle Scholar
  • 31 Sainte-Laudy J., Boujenaini N., Belon P. Confirmation of biological effects of high dilutions. Effects of submolecular concentrations of histamine and 1-, 3-and 4-methylhistamines on human basophil activation. Inflamm Res 2008; 57 (Suppl. 01) S27-S28.
    CrossrefPubMedGoogle Scholar
  • 32 Sainte-Laudy J., Boumediene A., Touraine F., Orsel I., Brianchon C., Bonnaud F. et al. Use of both CD63 up regulation and IgE down regulation for the flow cytometric analysis of allergen induced basophil activation. Definition of an activation index. Inflamm Res 2007; 56: 291-296.
    CrossrefPubMedGoogle Scholar
  • 33 Sainte-Laudy J., Belon P. Use of four different flow cytometric protocols for the analysis of human basophil activation. Application to the study of the biological activity of high dilutions of histamine. Inflamm Res 2006; 55 (Suppl. 01) S23-S24.
    CrossrefPubMedGoogle Scholar
  • 34 Sainte-Laudy J., Belon P. Improvement of flow cytometric analysis of basophil activation inhibition by high histamine dilutions. A novel basophil specific marker: CD 203c. Homeopathy 2006; 95: 3-8.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 35 Hadjaj B., Cherruault Y., Sainte Laudy J. Control of basophil degranulation. Int J Biomed Comput 1993; 32: 151-159.
    CrossrefPubMedGoogle Scholar
  • 36 Sainte-Laudy J. Modulation of allergen and anti-IgE induced human basophil activation by serial histamine dilutions. Inflamm Res 2000; 49 (Suppl. 01) S5-S6.
    CrossrefPubMedGoogle Scholar
  • 37 Brown V., Ennis M. Flow-cytometric analysis of basophil activation: inhibition by histamine at conventional and homeopathic concentrations. Inflamm Res 2001; 50 (Suppl. 02) S47-S48.
    PubMedGoogle Scholar
  • 38 Lorenz I., Schneider E.M., Stolz P., Brack A., Strube J. Sensitive flow cytometric method to test basophil activation influenced by homeopathic histamine dilutions. Forsch Komplementarmed Klass Naturheilkd 2003; 10: 316-324.
    PubMedGoogle Scholar
  • 39 Lorenz I., Schneider E.M., Stolz P., Brack A., Strube J. Influence of the diluent on the effect of highly diluted histamine on basophil activation. Homeopathy 2003; 92: 11-18.
    Article in Thieme ConnectPubMedGoogle Scholar
  • 40 Guggisberg A.G., Baumgartner S.M., Tschopp C.M., Heusser P. Replication study concerning the effects of homeopathic dilutions of histamine on human basophil degranulation in vitro . Complement Ther Med 2005; 13: 91-100.
    CrossrefPubMedGoogle Scholar
  • 41 Chirumbolo S., Brizzi M., Ortolani R., Vella A., Bellavite P. Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study. Inflamm Res 2009; 58: 755-764.
    CrossrefPubMedGoogle Scholar