Amelioration of root-knot disease of lady's finger plants by potentized Cina and Santonin
Received02 November 2004
revised20 May 2005
accepted10 April 2006
26 December 2017 (online)
Lady's finger plants (Hibiscus esculentus), grown in pots, were inoculated with the second-stage larvae (76±6) of root-knot nematodes Meloidogyne incognita, starting 7 days later they were treated with Cina 30c, Santonin 30c or Ethanol 30c by foliar spray for 10 consecutive days. The drugs in 90% ethanol were diluted with distilled water 1:1000 before application on plants. Thirty days after the last treatment the plants were uprooted. Cina 30c and Santonin 30c reduced nematode infestation of plants significantly in terms of root-gall number, root-protein content and nematode population in roots. Santonin 30c reduced root water content. Santonin 30c may have influenced the water channel proteins of root tissues thereby altering the water contents of roots. The reduced water content in roots might have adversely affected the root-knot nematodes and thus reduced nematode infestation. Ethanol 30c also has some effect on treated plants.
- 1 Semblat JP, Bongiovanni M, Wajnberg E, Dalmasso A, Abad P, Castagnone-Sereno P. Virulence and molecular diversity of parthenogenetic root-knot nematodes. Meloidogyne spp. Heredity 2000; 84: 81–89.
- 2 Trudgill D.L., Blok V.C. Apomictic, polyphagous root-knot nematodes: exceptionally successful and damaging biotrophic root pathogens. Annu Rev Phytopathol 2001; 39: 53-77.
- 3 Sukul N.C. Plants antagonistic to plant parasitic nematodes. Indian Rev Life Sci 1992; 12: 23-52.
- 4 Luc M., Bridge J., Sikora R.A. Reflections on nematology in sub-tropical and tropical agriculture. in: Luc M., Sikora R.A., Bridge J. Plant Parasitic Nematodes in Sub-tropical and Tropical Agriculture. 1993. Wallingford, UK: CAB International; xi-xvii.
- 5 McClure M.A. Biological control of nematodes. in: Baker R.R., Dunn P.E. New Directions in Biological Control: Alternatives for Suppressing Agricultural Pest and Diseases. 1990. New York: Wiley-Liss Inc; 169-255.
- 6 Atkins S.D., Hidalgo-Diaz L., Kalisz H., Mauchline T.H., Kerry B.R. Development of a new nematode strategy for the control of root-knot nematodes (Meloidogyne spp) in organic vegetable production. Pest Manage Sci 2003; 59: 183-189.
- 7 Tzortzakakis E.A., Petsas S.E. Investigation of alternatives to methyl bromide for management of Meloidogyne javanica on green house grown tomato. Pest Manage Sci 2003; 59: 1311-1320.
- 8 Siddiqui I.A., Shaukat S.S. Trichoderma harzianum enhances the production of nematicidal compounds in vitro and improves biocontrol of Meloidogyne javanica by Pseudomonas fluorescence in tomato. Lett Appl Microbiol 2004; 38: 169-175.
- 9 Tahseen Q., Clark I.M., Atkins S.D., Hirsch P.R., Kerry B.R. Impact of the nematophagous fungus Pochonia chlamydosporia on nematode and microbial populations. Commun Agric Appl Biol Sci 2005; 70: 81-86.
- 10 Sukul N.C. Biopesticides in nematode control. in: Dhaliwal G.S., Kansal B.D. Management of Agricultural Pollution in India. 1994. New Delhi: Commonwealth publishers; 248-269.
- 11 Sukul N.C., Sukul A. Potentized Cina reduces root-knot disease of cowpeas. Environ Ecol 1999; 17: 269-273.
- 12 Sukul N.C., Sinhababu S.P., Datta S.C., Nandi B., Sukul A. Nematotoxic effect of Acacia auriculiformis and Artemisia nilagirica against root-knot nematodes. Allelopathy J 2001; 8: 65-72.
- 13 Lowry O.H., Rossebrough N.J., Farr A.R., Randall R.J. Protein measurement with Folinphenol reagent. J Biol Chem 1951; 193: 265-275.
- 14 Chatterjee A., Sukul N.C. Total protein of galled roots as an index of root-knot nematode infestation of lady's finger plants. Phytopathology 1981; 31: 372-374.
- 15 Das P., Sukul N.C. The influence of inoculum density of root-knot nematodes, soil type and irrigation on the growth and root gall formation of tomato plants. Visva-Bharati J Res 1984; 5: 158-192.
- 16 Sukul N.C., Sukul A. High Dilution Effects: Physical and Biochemical Basis. Dordrecht: Kluwer Academic Publishers; 2004.
- 17 Nelson D.L., Cox M.M. Lehninger Principles of Biochemistry. 3rd edn. New York: Macmillan Worth Publishers; 2000. p 46.
- 18 Saxena S.K. Nematode infestation in vegetables. in: Swarup G., Dasgupta D.R. Plant Parasitic Nematodes of India: Problems and Progress. 1986. New Delhi: Indian Agricultural Research Institute; 312-327.