Homeopathy 2003; 92(04): 195-202
DOI: 10.1016/j.homp.2003.08.014
Original Paper
Copyright ©The Faculty of Homeopathy 2003

Effects of homeopathic arsenic on tobacco plant resistance to tobacco mosaic virus.

Theoretical suggestions about system variability, based on a large experimental data set
L Betti
1  Department of Agro-Environmental Science and Technology, Faculty of Agriculture, Bologna University, Italy
,
L Lazzarato
1  Department of Agro-Environmental Science and Technology, Faculty of Agriculture, Bologna University, Italy
,
G Trebbi
1  Department of Agro-Environmental Science and Technology, Faculty of Agriculture, Bologna University, Italy
,
M Brizzi
2  Department of Statistical Sciences, Faculty of Statistical Sciences, Bologna University, Italy
,
GL Calzoni
3  Department of Evolutionary Experimental Biology, Faculty of Science, Bologna University, Italy
,
F Borghini
4  Association for Medicine Applied to Italian Research on Water, Roma, Italy
,
D Nani
5  Italian Anthroposophical Medical Group, Milano, Italy
› Author Affiliations
Further Information

Publication History

Received10 March 2003
revised19 June 2003

accepted11 August 2003

Publication Date:
22 December 2017 (online)

Abstract

Context: This research aimed at verifying the efficacy of homeopathic treatments by plant-based bioassays, which may be suitable for basic research, because they lack placebo effects and provide large datasets for statistical analyses.

Objective: To evaluate the effects of homeopathic treatments of arsenic trioxide (As2O3) on tobacco plants subjected to tobacco mosaic virus (TMV) inoculation as biotic stress.

Design: Blind, randomized experiment using tobacco leaf disks.

Materials and methods: Tobacco plants (Nicotiana tabacum L. cultivar Samsun) carrying the TMV resistance gene N. TMV inoculated leaf disks were floated for 3 days in the following:

Distilled water (control)

H2O 5 and 45 decimal and centesimal potencies

As2O3 5 and 45 decimal and centesimal potencies

The main outcome measures is the number of hypersensitive lesions observed in a leaf disk.

Results: Homeopathic treatments of arsenic induce two effects on the plant: (i) increased resistance to TMV; (ii) decrease variability between experiments (system variability).

Conclusions: In this experimental model two actions of homeopathic treatment were detected: decrease in system variability and enhancement of the natural tendency of the system towards an ‘equilibrium point’.

 
  • References

  • 1 Vallance AK. Can biological activity be maintained at ultra-high dilution? An overview of homeopathy, evidence and Bayesian Philosophy. J Altern Complement Med 1998; 4: 49–76.
  • 2 Swayne J. The starting point: pathography. Homeopathy 2002; 91: 22–25.
  • 3 Chibeni SS. On the scientific status of homeopathy. Br Hom J 2001; 90: 92–98.
  • 4 Baumgartner S, Heusser P, Thurneysen A. Methodological standards and problems in preclinical homeopathic potency research. Forsch Komplementärmed 1998; 5: 27–32.
  • 5 Brands M. Discussion: the magic of non-local concepts or the chair in the Rabbi's castle. Br Hom J 2000; 89: 141–145.
  • 6 Walach H. Magic of signs: a non-local interpretation of homeopathy. Br Hom J 2000; 89: 127–140.
  • 7 Hyland ME, Lewith GT. Oscillatory effects in a homeopathic clinical trial: an explanation using complexity theory, and implications for clinical practice. Homeopathy 2002; 91: 145–149.
  • 8 Betti L, Borghini F, Nani D. Plant models for fundamental research in homeopathy. Homeopathy 2003; 92: 129–130.
  • 9 Rubik B. Report of the status of research on homeopathy with recommendations for future research. Br Hom J 1989; 78: 86–96.
  • 10 Scofield AM. Homeopathy and its potential role in agriculture—a critical review. Biol Agric Hortic 1984; 2: 1–50.
  • 11 Khanna KK, Chandra S. Further investigations on the control of storage rot of mango, guava and tomato fruits with homeopathic drugs. Indian Phytopath 1989; 42: 436–440.
  • 12 Pongratz W, Endler PC. Reappraisal of a classical botanical experiment in ultra high dilution research. Energetic coupling in a wheat model. In: Endler PC, Schulte J (eds). Ultra High Dilution. Physiology and Physics. Dordrecht: Kluwer Academic Publishers, 1994, pp 19–26.
  • 13 Betti L, Brizzi M, Nani D, Peruzzi M. Effect of high dilutions of Arsenicum album on wheat seedlings from seeds poisoned with the same substance. Br Hom J 1997; 86: 86–89.
  • 14 Pongratz W, Nograsek A, Endler PC. Highly diluted agitated silver nitrate and wheat seedling development. Effect kinetics of a process of successive agitation phases. In: Schulte J, Endler PC (eds). Fundamental Research in Ultra High Dilution and Homeopathy. Dordrecht: Kluwer Academic Publishers, 1998, pp 143–154.
  • 15 Brizzi M, Nani D, Peruzzi M, Betti L. Statistical analysis of the effect of high dilutions of arsenic in a large dataset from a wheat germination model. Br Hom J 2000; 89: 63–67.
  • 16 Kombrink E, Schmelzer E. The hypersensitive response and its role in local and systemic disease resistance. Eur J Plant Pathol 2001; 107: 69–78.
  • 17 Ross AF. Systemic acquired resistance induced by localized virus infection in hypersensitive hosts. Virology 1961; 14: 340–358.
  • 18 Jabs T. Reactive oxygen intermediates as mediators of programmed cell death in plants and animals. Biochem Pharmacol 1999; 57: 231–245.
  • 19 Lam E, Kato N, Lawton M. Programmed cell death, mitochondria and the plant hypersensitive response. Nature 2001; 411: 848–853.
  • 20 Allan AC, Lapidot M, Culver JM, Fluhr R. An early tobacco mosaic virus-induced oxidative burst in tobacco indicates extracellular perception of the virus coat protein. Plant Physiol 2001; 126: 97–108.
  • 21 Numberger T, Scheel D. Signal transmission in the plant immune response. Trends Plant Sci 2001; 6: 372–379.
  • 22 Siegrist J, Orober M, Buchenauer H. β-aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salycylic acid. Physiol Mol Plant Pathol 2000; 56: 95–106.
  • 23 Oostendorp M, Kunz W, Dietrich B, Staub T. Induced disease resistance in plants by chemicals. Eur J Plant Pathol 2001; 107: 19–28.
  • 24 Orober M, Siegrist J, Buchenauer H. Mechanisms of phosphate-induced disease resistance in cucumber. Eur J Plant Pathol 2002; 108: 345–353.
  • 25 Hartley-Whitaker J, Ainsworth G, Meharg AA. Copper and arsenate induced oxidative stress in Holcus lanatus L. clones with differential sensitivity. Plant Cell Environ 2001; 24: 713–722.
  • 26 Meharg AA, Hartley-Whitaker J. Arsenic uptake and metabolism in arsenic resistant and nonresistant plant species. New Phytol 2002; 154: 29–43.
  • 27 Marathe R, Anandalakshmi R, Liu Y, Dinesh-Kumar SP. The tobacco mosaic virus resistance gene, N. Mol Plant Pathol 2002; 3: 167–172.
  • 28 Fraser RSS, Gerwitz A. A new physical assay method for tobacco mosaic virus using a radioactive virus recovery standard and the first derivative of the ultraviolet adsorption spectrum. J Virol Methods 1985; 11: 289–298.
  • 29 Betti L, Marini F, Marani F, Cuffiani M, Rabiti AL, Canova A. A TMV strain overcoming both Tm-2 and Tm-22 resistance genes in tomato. Phytopath Medit 1997; 36: 24–30.
  • 30 Galambos J, Simonelli I. Bonferroni-type Inequalities with Applications. New York: Springer-Verlag, 1996.
  • 31 Salthe SN. Evolving Hierarchical Systems. New York: Columbia University Press, 1985, pp 48–49.
  • 32 Welch GR, Smith HA. On the field structure of metabolic space-time. In: Mishra RK (ed). Molecular and Biological Physics of Living Systems. Dordrecht: Kluwer Academic Publishers, 1990, pp 53–87.
  • 33 Chauvet GA. Hierarchical functional organization of formal biological systems: a dynamical approach. Philos Trans R Soc London B 1993; 339: 425–444.
  • 34 Nani D. Sotto le Ceneri della Scienza. Venezia: Il Capitello del Sole, 1995, pp 124–128.
  • 35 Rey L. Thermoluminescence of ultra-high dilutions of lithium chloride and sodium chloride. Physica A 2003; 323: 67–74.
  • 36 Betti L, Trebbi G, Lazzarato L, et al. Non-thermal effects of IR carried microwaves on hypersensitive response of tobacco to TMV. Experimental support to a similarity between electromagnetism and homeopathy. J Altern Complement Med, submitted.
  • 37 Del Giudice E, Preparata G, Vitiello G. Water as a free electric dipole laser. Phys Rev Lett 1988; 61: 1085–1088.