Int J Angiol 1999; 8: S21-S24
DOI: 10.1007/BF01619845
Original Articles

© Georg Thieme Verlag KG Stuttgart · New York

Effects of magnesium dobesilate on nitric oxide synthase activity in endothelial cells

Christoph V. Suschek1 , Hubert Kolb2 , Victoria Kolb-Bachofen1
  • 1Researchgroup Immunobiology, Biomedizinisches Forschungszentrum, Medical Faculty, Heinrich-Heine-University of Düsseldorf, Düsseldorf, Germany
  • 2Diabetes Research Institute at the University of Düsseldorf, Düsseldorf, Germany
Presented at the 40th Annual World Congress, International College of Angiology, Lisbon, Portugal, July 1998.
Further Information

Publication History

Publication Date:
24 April 2011 (online)

Abstract

This study was done to determine the effects of the angioprotective agent dobesilate on expression and activity on the constitutive nitric oxide synthase (ecNOS) in resident endothelial cells, as well as of the inducible nitric oxide synthase (iNOS) in cytokine-activated endothelial cells, by recording, in culture supernatants, the concentrations of citrulline as a reaction product of both enzymes. In capillary, microvascular, and macrovascular endothelial cells, Mg dobesilate incubation (0.25–1 mM) for 24 hours led to a highly significant concentration-correlating increase in ecNOS activities. These increases were not due to iNOS expression, and with cytokine-activated endothelial cell cultures that do express iNOS only moderate effects with little or no concentration dependency were seen. Addition of the NOS inhibitor NG-monomethyl-L-arginine (NMA) significantly suppresses citrulline formation in all cultures as evidence for the enzyme specificity.

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