Cross validation of the Multiple Electrode Aggregometry

Jolanta M. Siller-Matula; Ghazaleh Gouya; Michael Wolzt; Bernd Jilma

doi:10.1160/TH08-10-0669

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 2009; 102(02): 397-403
DOI: 10.1160/TH08-10-0669

New Technologies, Diagnostic Tools and Drugs

Schattauer GmbH

Cross validation of the Multiple Electrode Aggregometry

A prospective trial in healthy volunteers

Jolanta M. Siller-Matula

¹Department of Clinical Pharmacology, Medical University of Vienna, Austria

,

Ghazaleh Gouya

¹Department of Clinical Pharmacology, Medical University of Vienna, Austria

,

Michael Wolzt

¹Department of Clinical Pharmacology, Medical University of Vienna, Austria

,

Bernd Jilma

¹Department of Clinical Pharmacology, Medical University of Vienna, Austria

› Institutsangaben

Abstract

Summary

Several test systems exist for assessment of platelet function in patients under clopidogrel or aspirin therapy. The objective was to cross-validate the Multiple Electrode Aggregometry (MEA) with three other methods used for determining platelet reactivity under treatment with clopidogrel and aspirin. Platelet function was assessed by the MEA, Vasodilator Stimulated Phosphoprotein (VASP) phosphorylation assay, Platelet Function Analyzer-100 (PFA-100) and the Cone and Platelet Analyzer. Measurements were performed in blood from nine healthy volunteers at baseline, 2, 4, 6 and 72 hours after clopidogrel and aspirin loading. The apparent effect size for clopidogrel and aspirin was greatest for the MEA: treatment induced a 19-fold difference in the arachidonic acid-induced platelet aggregation and an 11-fold difference in the adenosine diphosphate-induced platelet aggregation before/after treatment. For comparison, aspirin and clopidogrel induced only 2.0– to 2.6 -fold changes in other tests (VASP assay, Cone and Platelet Analyzer and PFA-100). Maximal effects were seen 2 hours after aspirin loading and shorter than 72 hours after clopidogrel loading. In conclusion, aspirin and clopidogrel produce stronger signals in the MEA compared to several other methods.

Keywords

Antiplatelet - aspirin - clopidogrel - platelet function - Multiplate Analyzer

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Referenzen

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