Factor V Leiden mutation is associated with enhanced arterial thrombotic tendency in lean but not in obese mice

Nobuo Nagai; Roger H. Lijnen; Audrey C. A. Cleuren; Frits R. Rosendaal; Berthe Van Hoef; Marc F. Hoylaerts; Bart J. M. Van Vlijmen

doi:10.1160/TH07-04-0306

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 2007; 98(04): 858-863
DOI: 10.1160/TH07-04-0306

Animal Models

Schattauer GmbH

Factor V Leiden mutation is associated with enhanced arterial thrombotic tendency in lean but not in obese mice

Nobuo Nagai

¹Center for Molecular and Vascular Biology, K. U. Leuven, Leuven, Belgium

,

Roger H. Lijnen

¹Center for Molecular and Vascular Biology, K. U. Leuven, Leuven, Belgium

,

Audrey C. A. Cleuren

²Einthoven Laboratory for Experimental Vascular Medicine

³Department of Thrombosis and Hemostasis and Leiden University Medical Center, Leiden, The Netherlands

,

Frits R. Rosendaal

²Einthoven Laboratory for Experimental Vascular Medicine

³Department of Thrombosis and Hemostasis and Leiden University Medical Center, Leiden, The Netherlands

⁴Department of Clinical Epidemiology, Leiden University Medical Center, Leiden, The Netherlands

,

Berthe Van Hoef

¹Center for Molecular and Vascular Biology, K. U. Leuven, Leuven, Belgium

,

Marc F. Hoylaerts

¹Center for Molecular and Vascular Biology, K. U. Leuven, Leuven, Belgium

,

Bart J. M. Van Vlijmen

²Einthoven Laboratory for Experimental Vascular Medicine

³Department of Thrombosis and Hemostasis and Leiden University Medical Center, Leiden, The Netherlands

› Author Affiliations

Abstract

Summary

The homozygous factorV Leiden mutation is associated with enhanced venous thrombotic risk. Obesity is a major risk factor for development of thrombotic cardiovascular disease. It was the objective of this study to investigate whether obesity affects the thrombotic risk associated with the mutation. Male mice with homozygous factor V Leiden mutation (Arg 504 to Gln) (FVQ/Q) and corresponding wild-type (WT) mice were kept on a standard fat diet (SFD) or high fat diet (HFD) for 14 weeks, and femoral artery thrombosis was induced by FeCl₃ treatment. As compared to SFD, HFD feeding for 14 weeks resulted in significantly higher body weight and fat mass associated with adipocyte hypertrophy, which were, however, similar for both geno types. In the FeCl₃-induced arterial thrombosis model, FVQ/Q mice kept on SFD had a 40% shorter occlusion time (p = 0.015) and 40% lower blood flow (p = 0.03), as compared to WT mice. However, on HFD the occlusion time and blood flow were not significantly different for both genotypes. This finding could not be explained by differential changes of coagulation factors in either genotype fed on SFD or HFD. In conclusion, on SFD, but not on HFD, the factorV Leiden mutation is associated with enhanced thrombotic tendency after FeCl₃ injury of the femoral artery, suggesting that in this model obesity rescues the increased thrombotic risk associated with the factorV Leiden mutation.

Keywords

Factor V Leiden - obesity - thrombosis

Full Text

References

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