Neuromuscular Blocking Action of Alkaloids from a Japanese Crude Drug “Shin-I” (Flos Magnoliae) in Frog Skeletal Muscle

Ikuko Kimura; Masayasu Kimura; Masao Yoshizaki; Kazuo Yanada; Shigetoshi Kadota; Tohru Kikuchi

doi:10.1055/s-2007-969876

Planta Medica, Table of Contents

Planta Med 1983; 48(5): 43-47
DOI: 10.1055/s-2007-969876

Research Articles

Neuromuscular Blocking Action of Alkaloids from a Japanese Crude Drug “Shin-I” (Flos Magnoliae) in Frog Skeletal Muscle

Ikuko Kimura¹ , Masayasu Kimura¹ , Masao Yoshizaki² , Kazuo Yanada³ , Shigetoshi Kadota³ , Tohru Kikuchi³

¹Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Toyama, Japan
²Institute of Herbary, Toyama Medical and Pharmaceutical University, Toyama, Japan
³Research Institute for Wakan-Yaku (Oriental Medicines), Toyama Medical and Pharmaceutical University, Toyama, Japan

Abstract

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Abstract

From the dried buds of Magnolia salicifolia MAXIM, d-coclaurine (1), d-reticuline (2), 1-N-methylcoclaurine (3) and yuzirine (4) were isolated. Fifty percent inhibitory doses (µM) in acetylcholine (ACh, 27.5 µM)-induced contraction of frog skeletal muscle were as follows: d-tubocuraine (d-TC) 3.09, 2 93.2, 3 211, 1 467 and 4 more than 945. 4 (94.5 and 315 µM) increased the ACh-induced contraction. Indirectly stimulated twitching of frog nerve-muscle preparations were completely blocked by the following doses (µM): d-TC 1.47, 1 31.1, 2 239 and 3 more than 257. 1, 2 and 3 did not or only slightly block directly stimulated twitching. In contrast 271 µM 4 blocked the directly stimulated twitching.

Key Word Index

Magnolia salicifolia - Magnoliaceae - Coclaurine - Reticuline - Methylcoclaurine - Neuromuscular blocking action

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