Summary
Nephropathy is common among the diabetic population. However, the molecular mechanisms
by which hyperglycemia can cause alteration in kidney structure and function is not
known. In this study, we examined the effect of high glucose levels on cellular growth
and membrane lpid peroxidation in Vero cells, an African green monkey kidney cell
line. Cell growth was assessed by a tetrazolium salt reduction test (MTT); and lipid
peroxidation was measured by thiobarbituric acid reactivity. Our results show that
elevated levels of glucose (25,50 and 100mM) can cause up to 50% reduction in cell
growth in cultured Vero cells as compared with controls (8 mM). Also, we observed
a significant increase in the amount of oxidative damage in Vero cells cultured with
elevated glucose concentrations. This study demonstrates that hyperglycemia can cause
increased lipid peroxidation and affect the cellular proliferation in a monkey kidney
cell line.
Key words
Vero Cells - Cell Growth - High Glucose - Lipid Peroxidation
